Biopolym. Cell. 1986; 2(1):19-23.
Structure and Function of Biopolymers
Fragment K 2-3 of plasminogen molecule carries a lysine-binding site
- A. V. Palladin Institute of Biochemistry, Academy of Sciences of the Ukrainian SSR
Kiev, USSR
Abstract
Limited pepsin digestion of plasminogen fragment K 1—3 leads to the formation of the first kringle and structure which contains the second and third kringles (fragment K 2—3). The isolated fragments are characterized according to molecular masses and amino acid composition. Fragment K 2—3 is shown to be able of binding with lysine-Sepharose, which indicates the existence of a lysine-binding site in its structure.
Full text: (PDF, in Russian)
References
[1]
Colleti D. On the regulation and control of fibrinolysis. Thromb Haemostas. 1980; 43(1):77-89.
[3]
Wiman B, Wallen P. Activation of human plasminogen by an insoluble derivative of urokinase. Structural changes of plasminogen in the course of activation to plasmin and demonstration of a possible intermediate compound. Eur J Biochem. 1973;36(1):25-31.
[4]
Sottrup-Jensen L, Clayes H, Zajdel M et al. The primary structure of human plasminogen : isolation of two lysine-binding fragments and one "Mini-plasminogen" (M. W. 38000) by elastase catalyzed specific limited proteolysis. Progress in chemical fibrinolysis and thrombolysis. Eds. J. F. Davidson, R. H. Roman, M. M. Samama, P. S. Desnoyers. New York : Raven press, 1978, v. 3:191-209.
[5]
Castellino FJ. Recent advances in the chemistry of the fibrinolytic system. Chem Rev. 1981; 81(5):431-46.
[6]
Wiman B, Collen D. Molecular mechanism of physiological fibrinolysis. Nature. 1978;272(5653):549-50.
[7]
Wiman B, Wallen P. The specific interaction between plasminogen and fibrin. A physiological role of the lysine binding site in plasminogen. Thromb Res. 1977;10(2):213-22.
[8]
Trexler M, Vali Z, Patthy L. Structure of the omega-aminocarboxylic acid-binding sites of human plasminogen. Arginine 70 and aspartic acid 56 are essential for binding of ligand by kringle 4. J Biol Chem. 1982;257(13):7401-6.
[9]
Novokhatny VV, Kudinov SA, Privalov PL. Domains in human plasminogen. J Mol Biol. 1984;179(2):215-32.
[10]
Deutsch DG, Mertz ET. Plasminogen: purification from human plasma by affinity chromatography. Science. 1970;170(3962):1095-6.
[11]
Cummins P, Perry SV. The subunits and biological activity of polymorphic forms of tropomyosin. Biochem J. 1973;133(4):765-77.
[12]
Novokhatniy VV, Kudinov SA. Getting miniplazminogena and pieces 1-4 peptic proteolysis of human plasminogen. Dokl Akad Nauk SSSR. 1984; )5):62-5.