Biopolym. Cell. 2003; 19(6):553-557.
Bioorganic Chemistry
Application of enzyme biosensor base on pH-sensitive field transistors for determination of glucose concentration in potato juice
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
Abstract
A method for potato quality monitoring based on the determination of glucose concentration in potato juice using pH-sensitive field effect transistors with immobilized glucose oxidase has been proposed. Glucose concentration in potato juice was determined by a method of standard additions using the linear part of a calibration curve within dynamic range of 0.2—2 mM glucose concentration. To estimate the accuracy of glucose detection by the biosensor proposed, the measurements were also performed by a colorimetric method («Diagluc» enzymatic kit, Ukraine). A good correlation between these techniques is obtained.
Full text: (PDF, in Russian)
References
[1]
Pritchard M. Relationships of sugars to color of processed potatoes. Potato Facts Bull.-New York: Manitoba Agricult. and Food, publ., 1993: 4.
[2]
Tai G, Xiong XY. Selection for low glucose content in potato breeding populations. Northeast Potato Technology Forum materials. Seabrook, 2001: 118.
[3]
Sowokinos J. Choices for potatoes that chip directly from 42 F without reconditioning. Valley Potato Grower. 1999. 64, N 114: 14-17.
[4]
Wu G. Disease resistance conferred by expression of a gene encoding H2O2-Generating glucose oxidase in transgenic potato plants. The Plant Cell. 1995;7(9):1357–68.
[5]
Pat USA 5, 959, 180. DNA sequences from potato encoding solanidine UDP-glucose glucosyltransferase and use to reduce glycoalkaloids in solanaceous plants. Moehs C. P., Allen P., Rockhold E., Stapleton A., Friedman M., Belknap W.. Publ. 1999.
[6]
Soldatkin AP, El’skaya AV, Shul’ga AA, Netchiporouk LI, Nyamsi Hendji AM, Jaffrezic-Renault N, et al. Glucose-sensitive field-effect transistor with additional Nafion membrane. Anal Chim Acta. 1993;283(2):695–701.
[7]
Volotovsky VV, Patskovsky SV. Device for measuring of gate potential of ion-sensitive field effect transistor. Devices Exp. Tech. 1996; 3: 168.
[8]
Soldatkin AP, Boubryak OA, Starodub NF, El’skaya AV, Sandrovsky AK, Shul’ga AA, Strikha VI. Urease biosensor based on field effect transistor. Design features and performance in a model conditions. Electrochemistry (Moskow). 1993; 29(3):315-9.
[9]
Dzyadevich SV, Korpan YI, Arkhipova VN, Alesina MYu, Martelet C, El'Skaya AV, Soldatkin AP. Application of enzyme field-effect transistors for determination of glucose concentrations in blood serum. Biosens Bioelectron. 1999;14(3):283-7.
[10]
Kim D, Goldberg I, Judy J. A micromashined amperometric nitrate sensor. Proc. Annu. Res. Rev. of UCLA Electrical Engineering Department. Los-Angeles, 2003: 19-25.
[11]
Tretyakov NN, Koshkin EI, Makrushin NM. Physiology and biochemistry of agricultural plants. M.: Kolos, 2000; 640 p.
[12]
Przybyt M. Influence of anions on glucose electrode response: application to extending concentration range. Biosens Bioelectron. 1998;13(3-4):471-7.