Biopolym. Cell. 2003; 19(5):445-450.
Molecular Mechanisms of Differentiation
A role of tyrosine kinase Syk during neuronal differentiation of raurine embryonal carcinoma cells P19, stimulated by retinoic acid
1, 2Kukharskyy V. M., 2Draberova E., 1, 3Blame Ya. B., 2Draber P.
  1. Taras Shevchenko National University of Kyiv
    64, Volodymyrska Str., Kyiv, Ukraine, 01033
  2. Institute of Molecular Genetics of the ASCR
    1083, Videnska, 142 20 Prague 4, Czech Republic
  3. Institute of Cell Biology and Genetic Engineering, NAS of Ukraine
    148, Akademika Zabolotnogo Str., Kyiv, Ukraine, 03680


Using a culture of murine embryonal carcinoma cells stimulated by retinoic acid as a model of neuronal differentiation the changes in the tyrosine kinase Syk expression during neuronal differentiation have been shown. Study on the Syk tyrosine kinase distribution in both soluble and insoluble, enriched by elements of cytoskeleton, fractions of cells, has revealed first the endocellular redistribution of tyrosine kinase Syk during neuronal differentiation. A possible interaction of tyrosine kinase Syk with cytoskeleton elements is assumed.


[1] Yanagi S, Inatome R, Ding J, Kitaguchi H, Tybulewicz VL, Yamamura H. Syk expression in endothelial cells and their morphologic defects in embryonic Syk-deficient mice. Blood. 2001;98(9):2869-71.
[2] Yanagi S, Inatome R, Takano T, Yamamura H. Syk expression and novel function in a wide variety of tissues. Biochem Biophys Res Commun. 2001;288(3):495-8.
[3] Obergfell A, Eto K, Mocsai A, Buensuceso C, Moores SL, Brugge JS, Lowell CA, Shattil SJ. Coordinate interactions of Csk, Src, and Syk kinases with [alpha]IIb[beta]3 initiate integrin signaling to the cytoskeleton. J Cell Biol. 2002;157(2):265-75.
[4] Inatome R, Yanagi S, Takano T, Yamamura H. A critical role for Syk in endothelial cell proliferation and migration. Biochem Biophys Res Commun. 2001;286(1):195-9.
[5] Sada K, Takano T, Yanagi S, Yamamura H. Structure and function of Syk protein-tyrosine kinase. J Biochem. 2001;130(2):177-86.
[6] Tsujimura T, Yanagi S, Inatome R, Takano T, Ishihara I, Mitsui N, Takahashi S, Yamamura H. Syk protein-tyrosine kinase is involved in neuron-like differentiation of embryonal carcinoma P19 cells. FEBS Lett. 2001;489(2-3):129-33.
[7] Dr?ber P, Mal? P. Mutants of embryonal carcinoma cells defective in the expression of embryoglycan. Proc Natl Acad Sci U S A. 1987;84(16):5798-802.
[8] Dr?berov? E, Dr?ber P. A microtubule-interacting protein involved in coalignment of vimentin intermediate filaments with microtubules. J Cell Sci. 1993;106 ( Pt 4):1263-73.
[9] Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227(5259):680-5.
[10] Dr?ber P. Quantification of proteins in sample buffer for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using colloidal silver. Electrophoresis. 1991;12(6):453-6.
[11] Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC. Measurement of protein using bicinchoninic acid. Anal Biochem. 1985;150(1):76-85.
[12] Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979;76(9):4350-4.
[13] Dr?ber P, Dr?berov? E, Linhartov? I, Viklick? V. Differences in the exposure of C- and N-terminal tubulin domains in cytoplasmic microtubules detected with domain-specific monoclonal antibodies. J Cell Sci. 1989;92 ( Pt 3):519-28.
[14] B?rtek J, Viklick? V, Horejs? V, Verlov? H, Dr?ber P. Production and characterization of monoclonal antibodies against human transferrin. Folia Biol (Praha). 1984;30(2):137-40.
[15] Edd? B, Rossier J, Le Caer JP, Berwald-Netter Y, Koulakoff A, Gros F, Denoulet P. A combination of posttranslational modifications is responsible for the production of neuronal alpha-tubulin heterogeneity. J Cell Biochem. 1991;46(2):134-42.
[16] Laferri?re NB, MacRae TH, Brown DL. Tubulin synthesis and assembly in differentiating neurons. Biochem Cell Biol. 1997;75(2):103-17.
[17] Faruki S, Geahlen RL, Asai DJ. Syk-dependent phosphorylation of microtubules in activated B-lymphocytes. J Cell Sci. 2000;113 ( Pt 14):2557-65.