Biopolym. Cell. 2003; 19(5):436-439.
Structure and Function of Biopolymers
Trp144 as a fluorescence probe for investigation of the C-module rapid conformation dynamics in eukaryotic tyrosyle-tRNA synthetase
1Kordysh M. A., 1Odynets K. A., 1Kornelyuk A. I.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


The spectra of fluorescence of the tyrosyle-tRNA synthetase C-module have been obtained. The microsurrounding of a tryptophan residue has been characterized using the methods of fluorescence spectroscopy and computational modeling. The rapid conformation dynamics of the C-module in nanosecond time scale is revealed using the fluorescence quenching with acrylamide and Cs ions.


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