Biopolym. Cell. 2003; 19(3):257-261.
http://dx.doi.org/10.7124/bc.000659
Genome and Its Regulation
Usage of ISSR and SSR-PCR DNA-marker to Ppd genes
1Balashova I. A., 2Feit V. I., 1Sivolap Yu. M.
  1. South Biotechnological Center of Plant Cultivation
    Ovidiopol'skaya dor., 3, Odessa, Ukraine, 65036
  2. Plant breeding and Genetics Institute, National Centre of Seed and Cultivar Investigation
    Ovidiopol'skaya dor., 3, Odessa, Ukraine, 65036

Abstract

SSR- and ISSR-analyses were used for creating a marker to genes responsible for the sensitivity to photoperiod, in particular, the genes PpdDIa and PpdBIa. The gene marker to PpdBIa is a polymorphic SSR-apticon of size 180 b. p. The gene marker to PpdDIa is nulle-allelic 350(–). The genotypes homozygous on PpdDIa are identified by the absence of amplification product of size 350 b. p.
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References

[1] Stelmakh AF. The role of genetic systems in ontogenetic adaptation of soft wheat. Ecological genetics and evolution. Chisinau: Shtiintsa, 1987;146-61.
[2] Goncharov NP. Genetic control of the photoperiodic response in wheat. S. H. biologiia. 1986; 11:84-90.
[3] Worland AJ, B?rner A, Korzun V, Li WM, Petrov?c S, Sayers EJ. The influence of photoperiod genes on the adaptability of European winter wheats. Euphytica. 1998;100(1/3):385–94.
[4] Fait VI, Stelmakh AF. Identification of genotypes Ppd some varieties of winter wheat. Ahroekolohiya i biotekhnolohiya. 1998;(2):189-94.
[5] Rokitsky P. F. Biological statistics. Minsk: Vysch Shkola, 1973 319 p.
[6] Sivolap YuM, Kalendar' RN, Netsvetaev VP. Use of polymerase chain reaction products to map the barley (Hordeum vulgare L.) Genome. Genetika. 1997; 33(1):53-9.
[7] Goncharov NP. Genetic control of photoperiodic response of soft spring wheat in connection with selection for earliness: Auth Thesis. ... kand biol nauk. Leningrad, 1986; 18 p.
[8] Malyshev SV, Kartel' NA. [Molecular markers in genetic mapping of plants]. Mol Biol (Mosk). 1997;31(2):197-208.
[9] R?der MS, Korzun V, Gill BS, Ganal MW. The physical mapping of microsatellite markers in wheat. G?nome. 1998;41(2):278–83.
[10] Liu ZW, Biyashev RM, Maroof MA. Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theor Appl Genet. 1996;93(5-6):869-76.