Biopolym. Cell. 2003; 19(3):252-256.
Cell Biology
Novel cell line with multiple markers, derivative of CHO-K1
1Kordyum V. A., 1Toporova E. K., 1Okunev O. V., 1Pokholenko Y. A., 1Suchorada E. M., 1Ruban T. A., 1Andrienko V. I., 1Irodov D. M.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


A cell line CHO-K1 /pro-has been consecutively transfected with plasmids pEGFP-C1 and pTR-GT60. A subline with genotype pro, neo+ , gfp+ , tkhv+ , hph+ has been obtained after selection in the selective media. The cells are auxotrophic mutants deficient in proline, resistant to antibiotics G-418 and hygromycin B, sensitive to Ganciclovir and produce greenish fluorescence when irradiated with long ultraviolet light.


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