Biopolym. Cell. 2003; 19(3):231-237.
Structure and Function of Biopolymers
Isolation and characterization of monoclonal antibodies against eukaryotic translation elongation factor 1A
1Pogrebnaya A. P., 2Markeyeva N. V., 3Lisogubov V. V., 4Fridberg A. M., 4Zabashnyi S. I., 3Usenko V. S., 1Filonenko V. V., 2Pogrebnoy P. V., 1Negrutskii B. S.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine
    45, Vasilkivska Str., Kyiv, Ukraine, 01022
  3. Pathomorphological Laboratory "BIONTEK"
    52A/88, Komsomol'ska Str., Dnipropetrovs'k, Ukraine, 49000
  4. Dnipropetrovsk Regional Clinical Hospital Mechnikov
    Sq. October, 14, Dnepropetrovsk, Ukraine, 49008


Eukaryotic translation elongation factor 1A (eEF1A) is one of the main components of the translation apparatus.The protein also fulfills a number of non-canonical functions not related directly to protein synthesis. In this work the homogenous elongation factor 1A was obtained from hen liver by several chromatographic procedures with different carriers. The purified protein was used to develop monoclonal antibodies. The anti-eEF1A antibodies obtained are species-unspecific, they recognize both eEF1A and eEF1A2 isoforms in ELJSA and immunoblotting. The antibodies have been successfully used for immunohistological studies on human tissues.


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