Biopolym. Cell. 2003; 19(3):224-230.
Structure and Function of Biopolymers
Study on modifying action of lectins on the toxic and mutagenic effects of Ni(II) ions in Bacillus subtills culture
1Karpova I. S., 1Koretskaya N. V.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


The sensitivity of B. subtilis rec+ and rec strains to the toxic action of Ni(II) ions after pretreatment of cells with lectins of plant (ConA, LAA, PHA, STA, WGA, SNA, VAA), animal (HPA) and bacterial (exolectin of B. subtilis) origin has been compared. Six lectins of different structure, physical/chemical properties and carbohydrate specificity demonstrated their ability to defense the cell partially or completely from the toxic action of Ni(II) in order ConA - STA > PHA-P - LAA > PHA-L > SNA. Only rec+ strain was sensitive to such protection. Three lectins did not show any protective effect. The untimutagenic effect of PHA-P and PHA-L preparations, being tested by the number of trp+ revertants, was found after pretreatment or postincubation with lectins but it was absent when lectin and NiCl2, were added to bacterial culture simultaneously. It is suggested that the protective and untimutagenic effects of lectins are realized via activation of the cell reparation system..


[1] Lukash LL. Mutagenesis and antimutagenesis are contrary directed processes determining the level of genetic variability and stability. Biopolym Cell. 1998; 14(6):500-511.
[2] Durnev AD, Seredenin SB. [The pharmacological problems of searching for and using antimutagens]. Vestn Ross Akad Med Nauk. 1993;(1):19-26.
[3] Lutsik AD, Detyuk AS, Lutsik MD. Lectin in histochemistry. Lviv: Lviv Univ. publ., 1989 144 p.
[4] Van Damme JM, Peumans W, Pustai A, Bardocz S. Handbook of plant lectins: properties and biomedical applications. Chichester etc.: John Wiley and Sons, 1998.—452 p.
[5] Borrebaeck CA, Carlsson R. Lectins as mitogens. Advances in lectin research / Ed. H. Franz. Berlin: VEB Volk and Gesundheit, 1989. Vol. 2:10—27.
[6] Lalchev SM. Cytogenetic evaluation antimutagenic activity of PHA in vivo. Sovrem Med. 1987; 38(12): 14-6.
[7] Lukash LL, Karpova IS, Miroshnichenko OS, Tikhonova TN, Lylo VV, Man'ko VG, Sukhorada EM, Golynskaia EL. [The effect of the lectin from Sambucus nigra inflorescences on spontaneous and alkylating agent-induced mutagenesis in mammalian somatic cells]. Tsitol Genet. 1997;31(5):52-60.
[8] Maksymchuk TP, Babenko TA. Features genotoxic and carcinogenic effects of metals. Eksp onkol. 1999. 12(4):3-9.
[9] Kada T, Sadaie Y, Sakamoto Y. Handbook of mutagenicity test procedures. Ed. B. J. Kilbey, M. legator, W. Nichols, C. Ramel. Amsterdam; New York; Oxford: Elsevier, 1984:13—31.
[10] Macewicz LL, Lukash LL. Heavy metals genetic activity in eukaryotic cells. Biopolym Cell. 2001; 17(1):5-19.
[11] Karpova IS, Koretskaya NV, Tykhonova TN, Pidpala OV, Lukash LL. Effect of Phaseolus vulgaris isolectius on spontaneous mutability of Bacillus subtilis rec+ and rec– strains. Biopolym Cell. 2001; 17(5):406-410.
[12] Polu?ktova EU, Prozorov AA. [Properties of Bacillus subtilis mutant rec149 with an increased frequency of defective phage induction]. Genetika. 1981;17(9):1588-92.
[13] Prozorov AA. Transformation of bacteria. M.: Nauka, 1988; 256 p.
[14] Bashkirov VI, Khasanov FK, Lakomova NM. Illegitimate recombination and amplification in Bacillus subtilis. Molecular. mechanisms genet. processes. M.: Nuka, 1990:237-43.
[15] Podgorskiy VS, Kovalenko EA., Simonenko IA. Lectins bacteria. Kiev: Naukova Dumka, 1992; 202 p.
[16] Koretska NV, Kochubey TO, Karpova IS. Study connection between structure and biological activity of iso lectins of Phaseolus vulgaris using different test systems. Visn. Kyyiv. nats. un-tu. Ser. Biolohiya. 2002;(36): 40-3.
[17] B?ssing A, Schietzel M. Apoptosis-inducing properties of Viscum album L. extracts from different host trees, correlate with their content of toxic mistletoe lectins. Anticancer Res. 1999;19(1A):23-8.
[18] Drake JW. A constant rate of spontaneous mutation in DNA-based microbes. Proc Natl Acad Sci U S A. 1991;88(16):7160-4.
[19] Lakhtin VM. [Molecular organization of lectins]. Mol Biol (Mosk). 1994;28(2):245-73.