Biopolym. Cell. 2003; 19(3):224-230.
http://dx.doi.org/10.7124/bc.000653
Structure and Function of Biopolymers
Study on modifying action of lectins on the toxic and mutagenic effects of Ni(II) ions in Bacillus subtills culture
1Karpova I. S., 1Koretskaya N. V.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

The sensitivity of B. subtilis rec+ and rec– strains to the toxic action of Ni(II) ions after pretreatment of cells with lectins of plant (ConA, LAA, PHA, STA, WGA, SNA, VAA), animal (HPA) and bacterial (exolectin of B. subtilis) origin has been compared. Six lectins of different structure, physical/chemical properties and carbohydrate specificity demonstrated their ability to defense the cell partially or completely from the toxic action of Ni(II) in order ConA - STA > PHA-P - LAA > PHA-L > SNA. Only rec+ strain was sensitive to such protection. Three lectins did not show any protective effect. The untimutagenic effect of PHA-P and PHA-L preparations, being tested by the number of trp+ revertants, was found after pretreatment or postincubation with lectins but it was absent when lectin and NiCl2, were added to bacterial culture simultaneously. It is suggested that the protective and untimutagenic effects of lectins are realized via activation of the cell reparation system..
Full text: (PDF, in Ukrainian)

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