Biopolym. Cell. 2003; 19(2):169-178.
Molecular and Cell Biotechnologies
Organ-specific and light-inducible expression of genes in transgenic plants: receiving and cloning root-, tuber- and leaf-specific promoters
1Ljoshina L. G., 1Medvedeva T. V., 1Bulko O. V., 1Galkin A. P., 1Kukhar V. P.
  1. Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine
    1, Murmans'ka Str., Kyiv, Ukraine, 02094


In this study the regulatory sequences ensuring organ-specific and light-dependent expression of several chimerical genes in bacterial and plant cells have been received and investigated. A fragment corresponding to the 5'-end sequence of the chloroplast ribosomal gene S12 (rpS12), a regulatory fragment of the patatin gene λpatl22, a promoter sequence of the gene of small subunit of ribulose-1,5-disphosphate carboxylase (rbcS3A) have been extracted and cloned in vectors containing marker genes. The organ-specific activity of the cloned promoters has been shown. The metallo-thioneine gene has been introduced into the vector constructed. The increased ability of transgenic plants to accumulate the ions of heavy metals in organ's cells with selective promoter-induced expression of the metallothioneine gene has been demonstrated.


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