Biopolym. Cell. 2003; 19(2):164-168.
Viruses and Cell
Discovery of lectin-like proteins in the tobacco and datura plants, infected by tobacco mosaic virus
- D. K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154, Academika Zabolotnogo Str., Kyiv, Ukraine, 03680
Abstract
The plant proteins of tobacco and datura able to interact with yeast mannans immobilized on spheron H-1000 have been studied by gel-filtration and affinity chromatography. The hypersensitive plants infected by TMV have been established to produce lectin-like proteins capable to interact as ligands with the yeast α( l,2)-α(l,3)-α(l,6)-connected mannan. Previous study showed this biopolymer can be involved in the hypersensitive virus-induced reaction, probably modulating the action of cellular glycopolymer-inductor(s). At the virus infection localisation the lectin-like proteins are suggested to convert into a soluble form and to function as a regulator owing to their specific interaction with the inducer(s).
Full text: (PDF, in Ukrainian)
References
[1]
Sharon I, Lis Kh. Carbohydrates in cell recognition. V mire nauki. 1993; 2(3):104—12.
[2]
Lyubimova AV, Salkova EG. Lectin-carbohydrate interactions in relationships plant-pathogen. Prikl Biokhim Mikrobiol. 1988; 24(5):595—606.
[3]
Albersheim P, Darvil G. Oligosaccharine. Sci Amer. 1985;25(3):44—50.
[4]
Sundaresan RVS, Kimmins WC. Effect of virus infection on the cell wall composition of lesions on lesion host Phaseolus vulgaris. Ann Bot. 1981;47(2):287-9.
[5]
Fritig B, Kauffman S, Dumas S, Geoffroy P, Kopp M, Legrand M. Mechanism of hypersensitivity reaction in plants. Plant Resistance to Viruses (Ciba Fund. Symp. 133). Chichester; New York: Wiley J. & Sons, 1987:92-108.
[6]
Wieringa-Brants DH, Dekker WC. Induced resistance in hypersensitive tobacco against tobacco mosaic virus by injection of intercellular fluid from tobacco plants with systemic acquired resistance. J Phytopathol. 1987;118(2):165–70.
[7]
Modderman PW, Schot CP, Klis FM, Wieringa-Brants DH. Acquired resistance in hypersensitive tobacco against tobacco mosaic virus, induced by plant cell wall components. J Phytopathol. 1985;113(2):165–70.
[8]
Kovalenko OG, Telegeeva TA, Shtakun AV, Pogorila ZO. Influence of some mono- and polysaccharides on localization of virus infection and on induced virus resistance in plants. Biopolym Cell. 2000; 16(1):53-59.
[9]
Kovalenko AG. Phytolectins: a possible base of antiviral activity of carbohydrate polymers in the hypersensitive host plant. Recent Results in Plant Virology: Symp. held in the Castle Reinhardsbrunn (DDR, March 23rd—March 27th, 1986): Summaries. Berlin, 1986: 50—51.
[10]
Kovalenko AG. Protein-carbohydrate interaction in therealization of plant resistance to viruses. Mikrobiol Zh. 1993; 55(6):74-91.
[11]
Kovalenko AG, Grabina TD, Kolesnik LV, Didenko LF, Oleschenko LT, Olevinskaya ZM, et al. Virus resistance induced with mannan sulphates in hypersensitive host plants. J Phytopathol. 1993;137(2):133–47.
[12]
Kovalenko AG, Yamskov IA. Yeast mannans as affinity sorbents for lectins. Book of Abstrs. 15th Int. Lect. Conf. Szeged, 1993; 54.
[13]
Kovalenko AG, Grabina TD, Kovalenko EA, Pilipenko VG. Induction of resistance to tobacco mosaic virus and changes in the composition of soluble proteins in tobacco. Mikrobiol Zh. 1987; 49(3):80-6.
[14]
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-54.
[15]
Hughes RC. Glycoproteins. London ; New York : Chapman and Hall, 1983. 95 p
[16]
Kovalenko AG, Shashkov AS, Vasiljev VN, Telegeeva TA. The structural features and biological activity of Candida spec. yeast mannans. Biopolym Cell. 1995; 11(3-4):88-95.
