Participation of nitrogen bases in the tertiary folding of tRNALeu from Thermus thermophilus

Kovalenko, O. P.; Kriklivyi, I. A.; Tukalo, M. A.

doi:10.7124/bc.000647

Biopolym. Cell. 2003; 19(2):151-156.

http://dx.doi.org/10.7124/bc.000647

Structure and Function of Biopolymers

Participation of nitrogen bases in the tertiary folding of tRNA^Leu from Thermus thermophilus

1Kovalenko O. P., 1Kriklivyi I. A., 1Tukalo M. A.

Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Reactivity of bases in tRNA^Leu from T. thermophilus has been studied by chemical modification methods. Obtained results suggest that molecules of tRNA^Leu have a canonical L-form in solution. Triple interactions (8–14)-21, (13–22)-9 and (15–48)-20a arc observed in the core. The latter two interactions represent the characteristic feature of the class II tRNA tertiary structures. Different reactivity of bases 15 and 21 in tRNA^Leu and tRNA^Ser from T. thermophilus indicates differences in the core tertiary structure of these tRNAs.

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