Biopolym. Cell. 2002; 18(6):534-539.
Molecular Biomedicine
Obtaining and characteristics of human cells at early haemopoiesis stages when cultured in vitro
1Vasylovska S. V., 1Votyakova I. A., 2Lukash L. L., 2Matsuka G. H.
  1. Medical Center of tissue and cell therapy "Embriotech"
    5, Verkhnya Str., Kyiv, Ukraine, 01133
  2. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


Data on quantitative and qualitative characteristics of haemopoietic cell populations of 5–12 weeks of gestation have been presented, as well as their changes after cryopreservation. The results obtained concern the content of granutocytic progenitors, which practically do not function in the developing organism in this period of embryo-genesis, however they contain receptors responding to colony-forming factors and coming into proliferation and differentiation when cultured in vitro.


[1] Amit M, Carpenter MK, Inokuma MS, Chiu CP, Harris CP, Waknitz MA, Itskovitz-Eldor J, Thomson JA. Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture. Dev Biol. 2000;227(2):271-8.
[2] Chertkov L, Fridenshtein AYa. Cellular basis of hematopoiesis. M.: Meditsina, 1977; 272.
[3] Kelemen E, Calvo W, Fliedner TM. Atlas of human hemopoietic development. New York: Springer, 1979: 226.
[4] Moore MA, Metcalf D. Ontogeny of the haemopoietic system: yolk sac origin of in vivo and in vitro colony forming cells in the developing mouse embryo. Br J Haematol. 1970;18(3):279-96.
[5] Hole N. Embryonic stem cell-derived haematopoiesis. Cells Tissues Organs. 1999;165(3-4):181-9.
[6] Gerasimova LP. [Hematopoietic function of the liver in human ontogeny]. Gematol Transfuziol. 1984;29(11):46-50.
[7] Sharma S, Bhargave M, Kochupillai V. Morphological pattern of hematopoiesis in human fetal liver. Fetal Liver Transplantation. New York: Alan R. Liss. Inc., 1985: 167-171.
[8] Kelemen E, J?nossa M. Macrophages are the first differentiated blood cells formed in human embryonic liver. Exp Hematol. 1980;8(8):996-1000.
[9] Eridani S, Mazza U, Massaro P, La Targia ML, Maiolo AT, Mosca A. Cytokine effect on ex vivo expansion of haemopoietic stem cells from different human sources. Biotherapy. 1998;10(4):295–8.
[10] Bertoncello I. Status of high proliferative potential colony-forming cells in the hematopoietic stem cell hierarchy. Curr Top Microbiol Immunol. 1992;177:83-94.
[11] Gluzman DF, Abramenko IV, Sklyarenko LM, Nadgornaya VA. Hematopoietic stem cells and progenitor cells of myelopoiesis. Lab. diagnosis of hematologic diseases. Kiev: MORION, 1998:25-41.
[12] Barak Y, Karov Y, Levin S, Soroker N, Barash A, Lancet M, Nir E. Granulocyt-macrophage colonies in culture of human fetal liver cells: morphologic and ultrastructural analysis of proliferation and differentiation. Exp Hematol. 1980; 8(7):837-47.
[13] Gryschenko VI, Lobyntseva GS, Votyakova IA, Shereshkov SI. Hematopoietic fetal liver cells (embryogenesis, cryopreservation and transplantation). Kiev: Naukova DUmka. 1988; 192 p.
[14] Handbook of clinical and laboratory studies. Ed EA. Kost. M.: Meditsina, 1975; 383 p.
[15] Pike BL, Robinson WA. Human bone marrow colony growth in agar-gel. J Cell Physiol. 1970;76(1):77-84.
[16] Pluznik DH, Sachs L. The cloning of normal "mast" cells in tissue culture. J Cell Physiol. 1965;66(3):319-24.
[17] Salmon SE, Buick RN. Preparation of permanent slides of intact soft-agar colony cultures of hematopoietic and tumor stem cells. Cancer Res. 1979;39(3):1133-6.
[18] Frenkel' MA, Kharlamova LA, Shereshkov SI, Volkova MA. [Morphologic characteristics of colonies in agar cultures of hemopoietic cells in chronic myeloid leukemia]. Ter Arkh. 1982;54(8):116-20.
[19] Rakov AA, Blinova TS, Bubnov AN. [Committed granulomonocytic precursors in human embryonic organs]. Tsitologiia. 1981;23(4):427-32.
[20] Lucarelli G, Howard D, Stohlman F Jr. Regulation of erythropoiesis. XV. Neonatal erythropoiesis and the effect of nephrectomy. J Clin Invest. 1964;43:2195-203.
[21] Calwo V, Carbonel F. The development of liver granulopoiesis in the human fetus. Fetal Liver Transplantation. Amsterdam, Oxford, Princeton: Excerpta Medica, 1980: 14-19.
[22] Chertkov L, Fridenshtein AYa. Cellular basis of hematopoiesis. M.: Meditsina, 1977; 272.
[23] Gluzman DF. Diagnostic cytochemistry Leukemia. Kiev: Naukova Dumka. 1998. 215 p.
[24] Metcalf D. Regulation of granulocyte and monocate-macrophage proliferation by colony stimulating factor. Exp Hematol. 1974; 2(4):167-73.
[25] Messner HA, Fauser AA, Lepine J, Martin M. Properties of human pluripotent hemopoietic progenitors. Blood Cells. 1980;6(4):595-607.
[26] Gerasimova LP. [Colony-forming capacity of various hematopoietic and lymphoid organs in human embryo- and fetogenesis]. Probl Gematol Pereliv Krovi. 1980;25(5):45-8.
[27] Rakov AA, Blinova TS, Bubnov AN. [Committed granulomonocytic precursors in human embryonic organs]. Tsitologiia. 1981;23(4):427-32.