Biopolym. Cell. 2002; 18(5):452-454.
Short Communications
Influence of ion interactions on the conformation of ribozyme specific to HIV-1 tat-RNA in solution
1Markelova O. Yu., 1Buryanovsky L. N., 1Kitam O. E., 1Krasnova K. F., 1Shved A. D.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Using the method of partial nudease hydrolysis it was shown that the medium ion composition essentially influenced tertiary interactions in the ribozyme molecule and changed its spatial structure.

References

[1] Jackson WH Jr, Moscoso H, Nechtman JF, Galileo DS, Garver FA, Lanclos KD. Inhibition of HIV-1 replication by an anti-tat hammerhead ribozyme. Biochem Biophys Res Commun. 1998;245(1):81-4.
[2] Michienzi A, Conti L, Varano B, Prislei S, Gessani S, Bozzoni I. Inhibition of human immunodeficiency virus type 1 replication by nuclear chimeric anti-HIV ribozymes in a human T lymphoblastoid cell line. Hum Gene Ther. 1998;9(5):621-8.
[3] Trang P, Lee M, Nepomuceno E, Kim J, Zhu H, Liu F. Effective inhibition of human cytomegalovirus gene expression and replication by a ribozyme derived from the catalytic RNA subunit of RNase P from Escherichia coli. Proc Natl Acad Sci U S A. 2000;97(11):5812-7.
[4] Michienzi A, Cagnon L, Bahner I, Rossi JJ. Ribozyme-mediated inhibition of HIV 1 suggests nucleolar trafficking of HIV-1 RNA. Proc Natl Acad Sci U S A. 2000;97(16):8955-60.
[5] Zhang Y, Leibowitz MJ. Folding of the group I intron ribozyme from the 26S rRNA gene of Candida albicans. Nucleic Acids Res. 2001;29(12):2644-53.
[6] Wang YX, Lu M, Draper DE. Specific ammonium ion requirement for functional ribosomal RNA tertiary structure. Biochemistry. 1993;32(46):12279-82.
[7] Buryanovsky LN. Effect of pH, ionic strength, and ionic composition on efficiency of hammerhead ribozyme-mediated cleavage of the tat-RNA HIV-I in vitro. Biopolym Cell. 1997; 13(1):30-5.
[8] Markelova OYu, Buryanovsky LM, Kitam OE, Krasnova KF, Shved AD. Effect of medium ionic composition on cleavage activity and formation of complex between hammerhead ribozyme and short HIV-1 taf-RNA. Biopolym Cell. 2002; 18(4):301-6.
[9] Heus HA, Uhlenbeck OC, Pardi A. Sequence-dependent structural variations of hammerhead RNA enzymes. Nucleic Acids Res. 1990;18(5):1103-8.
[10] Woisard A, Fourrey JL, Favre A. Multiple folded conformations of a hammerhead ribozyme domain under cleavage conditions. J Mol Biol. 1994;239(3):366-70.