Biopolym. Cell. 2002; 18(5):429-435.
http://dx.doi.org/10.7124/bc.000622
Viruses and Cell
Comparison of capsid proteins of tobacco mosaic virus Ukrainian isolates and common strain after trypsin fragmentation
1Shevchenko T. P., 2Tkachenko T. Yu., 2Viter S. S., 2Radavsky Yu. L., 1Boyko A. L., 1Polischuk V. P.
  1. Taras Shevchenko National University of Kyiv
    64, Volodymyrska Str., Kyiv, Ukraine, 01033
  2. Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine
    1, Murmans'ka Str., Kyiv, Ukraine, 02094

Abstract

The comparative analysis of tobacco mosaic virus two Ukrainian isolates (TMVch and TMVsh) and common strain (TMV VI) was carried out by means of peptide mapping of capsid protein hydrolyzed by trypsin with the following analysis of tryptic fragments using homo- and heterospecific antisera. Peptide mapping results were not shown significant differences at the peptide maps of investigated proteins with the exception of fraction 7 that was absent at TMV Ul capsid protein elution profile. The analysis of each protein tryptic fractions using virusspecific antisera revealed certain differences at the reactions of antisera with some fractions. The amino acid sequence of peptide from fraction 12 TMVch capsid protein was established. This sequence coincides completely with the polypeptide sequence of TMV Ul capsid protein at the position 72–90, however the recognition of this fragment by antisera was varied.
Full text: (PDF, in Ukrainian)

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