Biopolym. Cell. 2002; 18(5):411-416.
Structure and Function of Biopolymers
Radiogenic alterations of RNA and DNA in rat spleen under various conditions of Irradiation
1Petryna L. G.
  1. Ivano-Frankivsk State Medical Academy
    2, Halytska Str., Ivano-Frankivsk, Ukraine, 76000


The content of nucleic acids in the Vistar line rat spleen has been found to change after a total single γ-irradiation at 1.0, 5.0 and 9.0 Gy doses of 0.001, 0.01, 0.1 and 1.0 Gy/min dose power. The degree of these alterations, their direction and extent of manifestation depend on the dose of irradiation. The experimental data show that the decrease in irradiation intensity prolongs the time of reaching an extreme point and decreases the effect level in this point. The correlation between the RNA and DNA content changes in animals caused by their irradiation has been discussed.


[1] Shevchenko VA, Pomerantseva MD. Genetic consequences of ionizing radiation. M.: Nauka, 1985. 279 p.
[2] Kuzin AM, Yurov SS, Shchelkaeva NV, Dedkova EN. Mutability in yeast population Sporobolomyces alborubescens Derx. with long-term chronic exposure to low doses of radiation. Radiobiologiia. 1993; 33(3):419-23.
[3] Burlakova EB, Goloshchapov AN, Zhizhina GP, Konradov AA. New aspects of of the effects of low intensity radiation. Radiats Biol Radioecol. 1999;39(1):26-34.
[4] E?dus LKh. The mechanism of the initiation of low-dose effects. Radiats Biol Radioecol. 1994;34(6):748-58.
[5] Eidus LKh. Extrapolation of dose-effect relationships for cytogenetic aberrations from high to low doses. Radiats Biol Radioecol. 1999;39(1):177-80.
[6] Zhizhina GP, Skalatskaia SI, Burlakova EB. The effect of low doses of ionizing radiation on the DNA in the spleen in the irradiation of mice. Radiats Biol Radioecol. 1994;34(6):759-62.
[7] Kisel'gof EI, Mushkacheva GS. Nucleic acid metabolism in rat spleen and liver following long-term repeated gamma-irradiation. Radiobiologiia. 1977;17(5):722-7.
[8] Lloyd DC, Edwards AA, L?onard A, Deknudt G, Natarajan A, Obe G, Palitti F, Tanzarella C, Tawn EJ. Frequencies of chromosomal aberrations induced in human blood lymphocytes by low doses of X-rays. Int J Radiat Biol Relat Stud Phys Chem Med. 1988;53(1):49-55.
[9] Trudolyubova MG. Quantification of RNA and DNA in subcellular fractions of animal cells. In: Modern methods in biochemistry. Ed VI Orekhovich. M.: Meditsina, 1977: 313-316.
[10] Bruserud Q, Moen T. Production of lymphokines in irradiated lymphoid cell population. Immunol Meth. 1984; 71: 175-84.
[11] Owen JJ. B-cell development. Progr. Immunol. New York; London: Acad, press, 1980: 303-14.
[12] Vereshchako GG, Khodosovskaia AM, Bulovatskaia IV, Konoplia EF. Effect of external irradiation with different intensity at 1 Gy dose on the DNA, RNA and total protein content in the testis and liver of rats. Radiats Biol Radioecol. 1999;39(5):557-62.
[13] Mushkacheva GS, Kisel'gof EI. Characteristics of nucleic acid biosynthesis in the activation of erythroid cell proliferation due to prolonged gamma irradiation. Radiobiologiia. 1983;23(3):363-6.
[14] Mushkacheva GS, Shorokhova VB. Effect of tritium oxide in a wide range of doses on the nucleic acid metabolism of the rat spleen. Radiobiologiia. 1989;29(4):554-7.