Biopolym. Cell. 2002; 18(4):356-358 .
Short Communications
Infrared absorption spectra of nucleic acids: the attenuated total reflection technique
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
Abstract
For the first time the attenuated total reflection (ATR) technique has been applied to obtain the IR absorption spectra of DNA and RNA. The procedure of removing NA films from a germanium prism has been worked through, thus a main obstacle in an appication of this technique to nucleic acids has been overcome.
Full text: (PDF, in Ukrainian)
References
[1]
Tsuboi M. Infrared and Raman spectroscopy. Basic principles in nucleic acid chemistry. Ed. P. O. P. Tso. New York, London: Acad, press, 1974. Vol. 1: 399-452.
[2]
Parker FS. Application of infrared, Raman, and resonance Raman spectroscopy in biochemistry. New York, London: Plenum press, 1983. 550 p.
[3]
Harrick NJ. Internal Reflection Spectroscopy. N.Y.: Interscience Publ., 1967
[4]
Yakutin VI, Strukov OG. Internal reflection spectroscopy. applications in chemistry and industry. Russ Chem Rev. 1972; 41 (8):692–709.
[5]
Zolotarev VM, Lygin VI, Tarasevich BN. Internal reflection spectra of surface compounds and adsorbed molecules. Russ Chem Rev. 1981,; 50 (1):14–31. http://dx.doi.org/ 10.1070/RC1981v050n01ABEH002541
[6]
Vigano C, Manciu L, Buyse F, Goormaghtigh E, Ruysschaert JM. Attenuated total reflection IR spectroscopy as a tool to investigate the structure, orientation and tertiary structure changes in peptides and membrane proteins. Biopolymers. 2000;55(5):373-80.
[7]
Hofer P, Fringeli UP. Structural investigation of biological material in aqueous environment by means of infrared-ATR spectroscopy. Biophys Struct Mech. 1979;6(1):67-80.
[8]
Rothschild KJ. FTIR difference spectroscopy of bacteriorhodopsin: toward a molecular model. J Bioenerg Biomembr. 1992;24(2):147-67.
[9]
Maleev VIa, Semenov MA, Gasan AI, Kashpur VA. [Physical properties of the DNA-water system]. Biofizika. 1993;38(5):768-90.
[10]
Semenov MA, Bol'bukh TV, Saga?dakova NN, Maleev VIa. [Hydration and structure of hemiprotonated polycytidylic acid in films]. Biofizika. 2000;45(1):32-9.
[11]
Sukhorukov BI, Sukhorukov GB, Shabarchina LI, Montrel MM. Optical properties and the structure of Langmuir-Blodget film of the complexes of nucleic acids with lipids and synthetic amphilic molecules. II. IR spectra, hydration and conformational state of DNA in a multilayer Langmuir film of DNA complex with octadecylamine. Biofizika. 1996; 41(5):1016-25.
[12]
Semenov MA, Gasan AI, Nolnukh TV, Maleeev VYa. Hydratation and the structural trabsitios of DNA from Micrococcus lysodeikticus in film. Biofizika. 1996;41(5):1007-15.
[13]
Semyonov MA, Sukhorucov BI, Maleev VYa, Shabarchina LI. Infared spectroscopic study of temperature anomaly of water sorbed on DNA. Biofizika. 1979; 24(2): 210-16.
[14]
Raha S, Merante F, Proteau G, Reed JK. Simultaneous isolation of total cellular RNA and DNA from tissue culture cells using phenol and lithium chloride. Gene Anal Tech. 1990;7(7):173–7.