Biopolym. Cell. 2002; 18(4):340-346.
http://dx.doi.org/10.7124/bc.000616
Bioorganic Chemistry
Interaction of cyanine dyes with nucleic acids. New (pyrido)(thio)trimethincyanine dye CCyan 40 for fluorescent labeling of oligonucleotides
1Yarmoluk S. M., 2Losytskyy M. Yu., 1Kovatska V. B., 1Tomachynski S. M., 2Ogul'chansky T. Yu., 1Kostenko O. M., 3Kurdyukov V. V., 3Tolmachev O. I.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Taras Shevchenko National University of Kyiv
    64, Volodymyrska Str., Kyiv, Ukraine, 01033
  3. Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine
    1, Murmans'ka Str., Kyiv, Ukraine, 02094

Abstract

The spectral-luminescent characteristics of the CCyan 40 carbo-cyanine dye in free state and in the presence of DNA and synthetic polynucleotides werp studied. The dye has high molar extinction coefficient (5.7·104 M–1 cm–1 ) and average quantum yield value (0.042). In the presence of DNA, poly(dGCldGC) and poly(dAldT) the quantum yield of CCyan 40 increases in 5.5, 3.8 and 1.8 times respectively. We recommend the dye CCyan 40 for the fluorescent labeling of oligonucleotides using the reaction, proposed by us earlier.
Full text: (PDF, in Ukrainian)

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