Biopolym. Cell. 2002; 18(4):283-286.
http://dx.doi.org/10.7124/bc.00060C
Structure and Function of Biopolymers
Amplification of randomly polymorphic DNA of American tobaccos from Petunioides subgenus
1Komarnytsky S. I., 1Komarnytsky I. K.
  1. Institute of Cell Biology and Genetic Engineering, NAS of Ukraine
    148, Akademika Zabolotnogo Str., Kyiv, Ukraine, 03680

Abstract

Amplification of randomly polymorphic DNA is used to study the relations within the species of the genus Nicotiana. The results obtained confirmed this approach to be reliable and useful for phylogenetic analysis within the genus. The identification of relationships of every species requires an additional analysis of the data obtained by other molecular and biological assays to postulate its exact origin.
Full text: (PDF, in Ukrainian)

References

[1] Borisjuk N, Borisjuk L, Petjuch G, Hemleben V. Comparison of nuclear ribosomal RNA genes among Solanum species and other Solanaceae. Genome. 1994;37(2):271-9.
[2] Komarnitskii IK, Komarnitskii SI. Size polymorphism of restricted fragments of rDNA intergenic spacer in some tobacco species. Tsitol Genet. 1996; 30(1):65-72.
[3] Tingey SV, del Tufo JP. Genetic analysis with random amplified polymorphic DNA markers. Plant Physiol. 1993;101(2):349-52.
[4] Sivolap YuM, Solodenko AE, Burlov VV. RAPD analysis of molecular-genetic polymprphism in sunflower Helianthus annuss. Genetika. 1998; 34(2):266-71.
[5] Yu Y-L, Lin T-Y. Construction of phylogenetic tree forNicotiana species based on RAPD markers. J Plant Res. 1997;110(2):187–93.
[6] Komarnytsky SI, Komarnytsky IK. Phylogenetic tree of the australian species of Nicotiana based on the random amplified polymorphic DNA. Biopolym Cell. 2001; 17(4):278-82.
[7] Goodspeed TH. The genus Niconiana. Massachusetts: Waltham, 1954.536 p.
[8] Komarnytsky SI. An attempt to combine morphological characters and nuclear ribosomal DNA (internal transcribed spacer) sequences in phylogenetic studies in the genus Nicotiana. Biopolym Cell. 1999; 15(5):383-9.
[9] Komarnyts'ky? SI, Komarnyts'ky? IK, Cox A, Parokonny? AS. [The evolution of the sequences of the internal spacer of nuclear ribosomal DNA for American species in the genus Nicotiana]. Tsitol Genet. 1998;32(3):69-76. Ukrainian.
[10] Murray MG, Thompson WF. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 1980;8(19):4321-5.
[11] Komarnitskii IK, Komarnitskii SI. Size polymorphism of restricted fragments of rDNA intergenic spacer in some tobacco species. Tsitol Genet. 1996; 30(1):65-72.
[12] Felsenstein J. PHYLIP (Phylogeny Inference Package), version 3.5. 1993.
[13] Salts Y, Herrmann RG, Peleg N, Lavi U, Izhar S, Frankel R, Beckmann JS. Physical mapping of plastid DNA variation among eleven Nicotiana species. Theor Appl Genet. 1984;69(1):1-14.