Biopolym. Cell. 2002; 18(3):232-236.
http://dx.doi.org/10.7124/bc.000603
Molecular and Cell Biotechnologies
Phenotype display of Escherichia coli BL21 (DE3) cell metabolism during growth in the media containing various types of carbon substrates
1, 2Slavchenko I. Yu., 2Boreyko E. V.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. PSRS "Biotechnolog"
    150, Akademika Zabolotnogo Str., Kyiv, Ukraine, 03680

Abstract

The E. coli strain BL21 (DE3) is widely applied for the synthesis of recombinant products using the bacteriophage T7 polymerase-base expression systems. The level of target protein production in E. coli cells has been recently shown to depend on the type of carbon source in the growth media and the producer metabolic properties, especially by acetate production. The growth of the strains and overproduction of recombinant proteins are inhibited by acetate. The growth, acid-generation and gas-forming during cultivation of E. coli BL2I (DE3) cells in the media, containing 13 various types of carbon substrates, have been tested in this work. It was found that BL21 (DE3) cells cannot utilize sucrose and inositol The least level of acid-generation is observed when glycerol is used as the carbon source.
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