Biopolym. Cell. 2002; 18(2):135-138.
http://dx.doi.org/10.7124/bc.0005F5
Development of the plate assay screening procedure for isolation of the mutants deficient in inactivation of peroxisomal enzymes in the yeast Yarrowia lipolytica
1Nazarko T. Y., 1Mala M. J., 1Sibirny A. A.
  1. Institute of Cell Biology, NAS of Ukraine
    14/16, Drahomanov Str., Lviv, Ukraine, 79005

Abstract

The amine oxidase (AMO) plate assay screening procedure for isolation of the mutants deficient in inactivation of peroxisomal enzymes in the yeast Y. lipolytica has been developed. The first tagged mutants affected in the peroxisomal AMO and isocitrate lyase inactivation were generated by the insertion of a zeta-URA3 mutagenesis cassette into the genome of a zeta-free and ura3 deletion mutant strain of Y. lipolytica.
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