Biopolym. Cell. 2001; 17(6):546-550.
Molecular and Cell Biotechnologies
The expression of human alpha-2b interferon in different strains of Escherichia coli
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
Abstract
The expression of human alpha-2b-interferon (IFN) in various strains of E. coli carrying the same recombinant plasmid plF-16 has been investigated. The level of IFN synthesis in different E. coli straines is shown to vary from electrophoretically undetectable amount to 35 % of the total cell proteins under the identical conditions of producer cultivation. The difference in the levels of interferon synthesis is not related to genetic instability of the plasmids.
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