Biopolym. Cell. 2001; 17(4):314-318.
Viruses and Cell
Influence of TMV and RNA TMV on culture of cells different origin
1Boyko A. L., 1Kyrjachenko S. S., 2Yudina O. Yu., 2Savtsova Z. D.
  1. Taras Shevchenko National University of Kyiv
    64, Volodymyrska Str., Kyiv, Ukraine, 01033
  2. R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine
    45, Vasilkivska Str., Kyiv, Ukraine, 01022


The assemblage of virus-like particles, which showed the infection on plants-indicators, has been observed after transfection RNA of TMV in OH-1 (the line is obtained from mouse lymphomas line A appeared after introduction of Bac. megaterium H) and PTP (the line is obtained from pig egg cells). The morphological changes of infected cells, which are correlated with accumulation virus-specific antibody in cells, were observed beginning from third day.


[1] Moskovets SN, Boiko AL, Zhukova LM. Tobacco Mosaic virus on the rose plats. Dopovidi Akad Nauk Ukr RSR. Ser B. 1971; (5):461-3.
[2] Chernyuk SO, Kiryachenko SS. Characterization of isolates 318 TMV plant reserve Ascania Nova and Kanev State Reserve. Introduction and conservation of plant diversity. 2000; (3):69-71.
[3] Boyko AL, Stepanujk SA, Garifulin OM. Sequencing analysis cDNA for C-terminus part of capsid protein for tomato TVM isolated from region with nuclear contamination. Biopolym Cell. 1996; 12(5):100-5.
[4] Boiko AL, Nosach LN, Tokarchuk LV, Dyachenko NS. Interaction of RNA of the essential-oil rose mosaic and tobacco mosaic virus strain with HeLa cells. Dopovidi Akad Nauk Ukr RSR. Ser B. 1981;(3):56-8.
[5] Zhdanov VM, Tijhonenko TI, Bocharov AF, Naroditskiĭ BA. Reproduction of tobacco mosaic virus in isolated rat liver mitochondria. Dokl Akad Nauk SSSR. 1971;199(4):944-7.
[6] Taykova NV, Ignatenko TO, Kiryachenko SS, Molchanets OV. Effect of TMV on functional activity of macrophages white mice. 2nd Intern. Conf. "Bioresources and viruses" (7—10 Sept 1998). Kyiv, 1998; 104.
[7] Eick D, Hermeking H. Viruses as pacemakers in the evolution of defence mechanisms against cancer. Trends Genet. 1996;12(1):4-6.
[8] Teodoro JG, Branton PE. Regulation of apoptosis by viral gene products. J Virol. 1997;71(3):1739-46.
[9] Savirva ZD, Grinevich YuA, Menek TA, Leonov YuI, Usach OM. Viral infections associated with cancer. Suchasni infektsiyi. 2000; (3): 70-88.
[10] Butenko ZA, Phylchenkov AA. Modern concept of viral oncogenesis: fundamental and applyied aspects. Exp Oncol. 2000; 22(3)239-45.
[11] Fil'chenkov AA, Stoika RS. Apoptosis and cancer. Kyiv, Morion, 1999; 184 p.
[12] Practicum on general virology. Ed. IG Atabekova. Moscow: Moscow State University Press, 1981; 192 p.
[13] Zatula DG, Zavalnyuk AK, Lisovenko GS, Syadro TA. Induction of tumors in mice with B megaterium H., having common antigenic determinants with malignant cells. Eksp Onkol. 1981; 3(5):48-50.
[14] Zatula DH, Likhtenshtein VE, Zmareva EN, Zavelevich MP, Verkhatskyi PP. Morphology of transplantable coultures of mirine lymphoma induced by Bac. megaterium H. Materials of III Ull-Union Conf. «Modern methods of morphological study in theoretical and practical oncology» Tbilisi, 1983 Vol. 1:118-9.
[15] Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: A laboratory manual.—New York: Cold Spring Harbor Lab. press, 1989:16.41—16.44.
[16] Gnutova RV. Serology and immunochemistry of plant viruses. Moscow: Nauka, 1993; 300 p.
[17] Kiselev NA. Electron microscopy of biological macromolecules. M.: Nauka, 1965; 146 p.
[18] Kudryavets YuI, Phylchenkov AA, Abramenko IV, Polischouk LZ, Slukvin II, Belous NI. Dynamics of apoptotic events induced in human leukemia U-937 cells by tumor necrosis factor. Eksperimentalnaya Onkologiya. 1996;18 (4):353-365.
[19] Osman TA, Buck KW. The tobacco mosaic virus RNA polymerase complex contains a plant protein related to the RNA-binding subunit of yeast eIF-3. J Virol. 1997;71(8):6075-82.