Biopolym. Cell. 2001; 17(4):292-297.
Structure and Function of Biopolymers
Investigation of aminomodified mica as a substrate for nucleic acids atomic force microscopy
1, 2Limansky A. P.
  1. Mechnikov Institute of Microbiology and Immunology NAMS of Ukraine
    14, Pushkinska Str., Kharkiv, Ukraine, 61057
  2. Department of Microbiology, Arizona State Univivesity
    Tempe, AZ 85287-2701 USA


Adhesion forces for aminomodified (AP) mica in aqueous solutions at different ionic strength (I) and pH were determined by atomic force microscopy (AFM). Adhesion force values have been decreased in direction I– 10 mM →I– 100 mM→ pH 11.2 for pair tip –AP-mica which was modified with 3-aminopropyltriethoxy silane (APTES) vapour. Stability of AP-mica surface was estimated by AFM imaging of linear DNA λ and supercoiled plasmid DNA pUC8 immobilized at AP-mica. Best DNA images were received at API mica, i. e. after mica modification with. APTES vapour in I hour.


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