Biopolym. Cell. 2001; 17(4):278-282.
Structure and Function of Biopolymers
Phylogenetic tree of the australian species of Nicotiana based on the random amplified polymorphic DNA
1Komarnytsky S. I., 1Komarnytsky I. K.
  1. Institute of Cell Biology and Genetic Engineering, NAS of Ukraine
    148, Akademika Zabolotnogo Str., Kyiv, Ukraine, 03680


To apply random amplified polymorphic DNA for analysis of phylogenetic relationships among australian species of the genus Nicotiana, we used 20 synthetic oligonucleotides as primers to examine intraspecific variation among 22 species of the genus. On the basis of the dendrogram constructed with maximal parsimony (Wagner) method, investigated species were subdivided into three major clusters, with basic species of the section localized in two of them, in a strict agreement with classical taxonomy of the section.


[1] Goodspeed TH. The genus Niconiana. Massachusetts: Waltham. 1954. 536 p.
[2] Merxmuller H, Battler KP. Nicotiana in der Afrikanischen Namibein Pflanzengeographisches and Phylogenetisches Ratsel. Mitt. Bot. Munchen. 1975; 12:91-104.
[3] Kostoff D. Cytogenetics of the genus Nicotiana. Sofia: State Printed House, 1941-1943. 1071 p.
[4] Tingey SV, del Tufo JP. Genetic analysis with random amplified polymorphic DNA markers. Plant Physiol. 1993;101(2):349-52.
[5] Burbidge NT. The australian species of Nicotiana L. (Solanaceae). Aust J Bot. 1960; 8(3): 342-395.
[6] Hsiao C, Chatterton NJ, Asay KH, Jensen KB. Molecular phylogeny of the Pooideae (Poaceae) based on nuclear rDNA (ITS) sequences. Theor Appl Genet. 1995;90(3-4):389-98.
[7] Baldwin BG. Molecular Phylogenetics of Calycadenia (Compositae) Based on ITS Sequences of Nuclear Ribosomal DNA: Chromosomal and Morphological Evolution Reexamined. Am J Bot. 1993;80(2):222-38.
[8] Komarnitski? SI, Komarnistki? IK, Cox A, Parokonny? AS. [Molecular phylogeny of the nuclear 5.8S ribosomal RNA genes in 37 species of Nicotiana genus]. Genetika. 1998;34(7):883-9.
[9] Komarnyts'ky? SI, Komarnyts'ky? IK, Cox A, Parokonny? AS. [The evolution of the sequences of the internal spacer of nuclear ribosomal DNA for American species in the genus Nicotiana]. Tsitol Genet. 1998;32(3):69-76.
[10] Komarnytsky SI. An attempt to combine morphological characters and nuclear ribosomal DNA (internal transcribed spacer) sequences in phylogenetic studies in the genus Nicotiana. Biopolym. Cell. 1999; 15(5):383-9.
[11] Yu Y-L, Lin T-Y. Construction of phylogenetic tree forNicotiana species based on RAPD markers. J Plant Res. 1997;110(2):187–93.
[12] Sivolap YuM, Solodenko AE, Burlov VV. RAPD analysis of molecular-genetic polymorphism in Sunflower Helianthus annuus. Genetika. 1998; 34(2):266-71.
[13] Welsh J, McClelland M. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 1990;18(24):7213-8.
[14] Murray MG, Thompson WF. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 1980;8(19):4321-5.
[15] Komarnitskii IK, Komarnitskii SI. Size polymorphism of restricted fragments of rDNA intergenic spacer in some tobacco species. Tsitol Genet. 1996; 30(1):65-72.
[16] Bahadur B, Farooqui SM. Seed and seed coat characters in australian Nicotiana II Solanaceae. Biology and systematics. Ed. W. G. D'Arcy. New York: Columbia Univ. press, 1986: 114-37.