Biopolym. Cell. 2001; 17(3):225-229.
Genome and Its Regulation
The physical mapping of the genome of MS2-induced DNA-containing P23-2 phage and detecting of MS2-specific sequences in this genome
1Miryuta N. Yu., 1Miryuta A. Yu., 1Pererva T. P.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


A physical map of the genome of MS2-induced DNA-containing P23-2 phage has been constructed. The order of HindiII, PstI, PvuII, BamHI, EcoRI, SmaI restriction enzymes sites has been determined. The size of the phage DNA is calculated to be about 40 kbp. The MS-2-specific sequences have been detected in this genome.


[1] Gren EYa. Regulatory mechanisms of RNA containing bacteriophages replication. Riga: Zinatne, 1974. 232 p.
[2] Pererva TP, Miryuta NYu, Miryuta AYu. MS2 phage lysogeny. Phage-specific RNA synthesis on cell DNA. Biopolym Cell. 1993; 9(1):45-50.
[3] Pererva TP, Mirjuta NYu, Mirjuta AYu, Woodmaska MI, Zherebtsova EN. Lysogeny by MS2 phage. Analysis of a recombinant plasmid containing MS2 RNA-like sequence. Biopolym Cell. 1995; 11(1):61-5.
[4] Pererva TP, Miriuta AJ, Woodmaska MI, Alekseenko IP. Lyzogeny by MS2 phage. Expression of MS2-specific information with segregants of unstable transducing P1- and ? phages. Biopolym Cell. 1996; 12(4):73-83.
[5] Pererva T. P. DNA-containing phages neutralizing with anti-MS2 serum. Biopolym Cell. 1998;14(6):549-52.
[6] Adams M. Bacteriophages. New York; London: im. Publ. Inc., 1951.
[7] Devos R, van Emmelo J, Contreras R, Fiers W. Construction and characterization of a plasmid containing a nearly full-size DNA copy of bacteriophage MS2 RNA. J Mol Biol. 1979;128(4):595-619.
[8] Maniatis T, Frisch EF, Sambrook J. Molecular cloning: A laboratory manual. New York: Cold Spring Harbor Lab. publ., 1982. 452 p.
[9] Fitch WM, Smith TF, Ralph WW. Mapping the order of DNA restriction fragments. Gene. 1983;22(1):19-29.
[10] Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975;98(3):503-17.
[11] Burrington MG, Seehafer J, Downer DN, Colter JS. Rescue of BKV from BKV-transformed hamster, rat, and mouse cells: correlation with levels of nonintegrated viral DNA. Virology. 1984;138(1):168-73.
[12] Domingo E, Mart?nez-Salas E, Sobrino F, de la Torre JC, Portela A, Ort?n J, L?pez-Galindez C, P?rez-Bre?a P, Villanueva N, N?jera R, et al. The quasispecies (extremely heterogeneous) nature of viral RNA genome populations: biological relevance--a review. Gene. 1985;40(1):1-8.