Biopolym. Cell. 2001; 17(2):160-165.
http://dx.doi.org/10.7124/bc.0005A7
Molecular and Cell Biotechnologies
Isolation of clones sensitive to bacteriophage λ from the phage resistant Escherichia coli strain
1Slavchenko I. Yu.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

A method of selection of the phage λ sensitive clones from the population of the bacteriophage λ resistant cells using the phage λcI857ApRTcR has been theoretically formulated and experimentally approved. The isolation was realized as follows. The phage λ resistant E. coli strain was infected by phage λ, which introduces antibiotic-resistance markers into the cell. The sensitive to phage λ vir and lysing at 43 В°C clones were isolated on the selective medium. The frequency of occurrence of such cells in the population was 10–6 . The presence of ts-mutation in the phage cI gene has allowed to cure the cells from phage λcI857ApRTcR during cultivation of these cells under non-selective conditions at increased temperature and to obtain the phage A sensitive clones originated from resistant E. coli strain. The method can also be used for the estimation of the E. coli populations heterogeneity by a given feature.
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