Biopolym. Cell. 2001; 17(1):80-84.
http://dx.doi.org/10.7124/bc.0005A1
Methods
Peptides and cyanine dyes. 2. Covalent labelling of amino acids and peptides by means of the reaction of pyrylium cyanine dyes with amines
1Kostenko O. M., 1Dmitrieva S. U., 1Yarmoluk S. M.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Here we propose a new amino-reactive pyrilium dye for the conjugation with peptides through N-terminal and N-ε-lysine's aminogroups. The reactivity of Cyan 39 towards eleven most interesting aminoacids has been investigated in triethylammonium acetate and sodium carbonate-bicarbonate buffers. The conjugation with 72-aminoacid model peptide has been carried out successfully.
Full text: (PDF, in Ukrainian)

References

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