Biopolym. Cell. 2000; 16(6):559-561.
http://dx.doi.org/10.7124/bc.000597
Molecular and Cell Biotechnologies
Isolation of site-specific endonuclease of Streptomyces sp. 48
1Lukyanchuk V. V., 1Sukhanoff S. M., 1Polishchuk L. V., 1Matselyukh B. P.
  1. D. K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
    154, Academika Zabolotnogo Str., Kyiv, Ukraine, 03680

Abstract

A method of isolation and purification of site-specific endonuclease of the II type Streptomyces sp. 48 has been worked out. ft has been determined that the Streptomyces sp. 48 lysate contains only one enzyme with site-specific activity. Maximum elution of the endonuclease was made by buffer with ionic strength of 350 mM NaCl. This method provides sufficient purification and concentration of this enzyme with preservation of its specific activity.
Full text: (PDF, in Ukrainian)

References

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