Biopolym. Cell. 2000; 16(5):409-412.
Cell Biology
Vitality of cells in culture after combined exposure to heavy metals and radiation
1Dudchenko T. N., 1Lavrenchuk G. I., 1Serkiz Ya. I., 2Zinchenko V. A.
  1. R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine
    45, Vasilkivska Str., Kyiv, Ukraine, 01022
  2. Institute of Oncology of Academy of Medical Sciences of of Ukraine
    Street. University, 33/43, Kiev, Ukraine, 03022


Combined influence of middleeffective concentrations of heavy metals (Pb, Ba, Cr, Ni, Cu) and irradiasion by X-rays in LD-50 dose on the indices of vitality of L-cells in culture has been investigated. Kinetic changes of indices are specific for different elements and depend on the level of cellular growth. It was shown that combined exposure to Cr, Pb and radiation on the level of intense cellular growth manifested sinergic or adaptative influence (it decreased growth of cells population, the mitotic index and increased the number of gigantic cells). Cu causes the contrary effect. Pb and Ba increase the mitotic index and the numberof gigantic cells on the stationary growth level.


[1] Spitkovski? DM. Concept of the effect of low doses of ionizing radiation on cells and its possible use in the interpretation of medical-biological consequences. Radiobiologiia. 1992;32(3):382-400.
[2] Serkiz J, Pintschuk VG, Pintschuk LB, Druzina NO, Puchova GG. Tschernobyl und seine Folgen. Radio-biologische Aspekte der Tschernobyler Katastrophe. Elbe: DNJEPR, 1994. Band 1. 212 p.
[3] Burlakova EB, Goloshchapov AN, Gorbunova NV, Gurevich SM, Zhizhina GP, Kozachenko AI, Konradov AA, Korman DB, Molochkina EM, Nagler LG, Ozerova IB, Skalatskaia SI, Smotriaeva MA, Tarasenko OA, Treshchenkova IuA, Shevchenko VA. The characteristics of the biological action of low doses of irradiation. Radiats Biol Radioecol. 1996;36(4):610-31.
[4] Medvedev SIu, Telichko FF, Gedeon VF. Distribution of ionizing radiation absorption in microstructures of a biosystem. Radiats Biol Radioecol. 1997;37(5):727-34.
[5] Baraboy VA, Tanzum TV, Kovalenko LS, Zinchenko VA, Lawentchuk GJ. Morphometric characteristic of cell culture L929 after the termic and radiation action. Biopolym Cell. 1999; 15(1):38-42.
[6] Handbook on the Toxicology of Metals. Eds L. Frieberg, G. F. Nordberg, B. V. Vourk. Amsterdam: Elsevier, 1979. 687 p.
[7] Maksimchuk TP, Babenko GA. Characteristics of genotoxic and carcinogenic action of metals. Eksp Onkol. 1990;12(4):3-9.
[8] Kavetsky RE, Serkyz YaI, Druzhina NA, Pinchouk VG. Chemiluminescence of irradiated animal blood plasma. J Biolumin Chemilumin. 1990;5(3):203-6.
[9] Liu D, Jiang W, Wang W, Zhai L. Evaluation of metal ion toxicity on root tip cells by the Allium test. Isr J Plant Sci. 1995;43(2):125–33.
[10] Dovgalyuk AI, Kalynyak TB, Blyum YAB. The toxic ffect of metal ions on the root growth and mitotic activity of onion (Allium cepa L.) cell. Dopovidi Nats Akad Nauk Ukrainy. 1998; (6):173-8.
[11] Bogomazov MIa. The effect of whole-body x-ray irradiation on the absorption of metals from the gastrointestinal tract in relation ot the degree of their oxidation. Gig Sanit. 1990;(9):59-61.
[12] Adams RLP. Cell Culture for Biochemists. Elsevier. North-Holland Biomedical Press. 1980; 292 p.
[13] Dudchenko TM, Lavrenchuk HI, Naboka MV, Serkiz IaI, Chebotar'ov IeIu. The cytotoxic effect of heavy metals on an L-cell culture. Tsitol Genet. 2000;34(3):55-61.