Biopolym. Cell. 2000; 16(5):339-344.
Structure and Function of Biopolymers
Search and characterization of melanoma antigens with the use of serological identification of antigens by recombinant expression cloning
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
- Division of Cell Regulatory Systems of O.V. Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine
14/16, Drahomanov Str., Lviv, Ukraine, 79005
- Ludwig Institute for Cancer Research
91 Riding house str., London, UK, WIP8BT
Ten positive clones nave been identified during immunoscreening of expressing cDNA libraries by autologous sera. One of the clones encoded for a fragment of Ki-67 protein have been subcloned and the protein expressed in bacteria. Heterologous screening of recombinant Ki-67 protein by allogenic sera have been performed. Statistical analysis indicated significant difference in the level of autoantibodies in cancer sera in comparison with normal ones. The data obtained can be useful for the understanding of the malignance processes, and for the diagnostic and therapeutic purposes as well
 Parmiani G. Tumor immunity as autoimmunity: tumor antigens include normal self proteins which stimulate anergic peripheral T cells. Immunol Today. 1993;14(11):536-8. PMID:8274196
 Pardoll DM. New strategies for enhancing the immunogenicity of tumors. Curr Opin Immunol. 1993;5(5):719-25.
 Robbins PF, Kawakami Y. Human tumor antigens recognized by T cells. Curr Opin Immunol. 1996;8(5):628-36.
 Brichard V, Van Pel A, W?lfel T, W?lfel C, De Plaen E, Leth? B, Coulie P, Boon T. The tyrosinase gene codes for an antigen recognized by autologous cytolytic T lymphocytes on HLA-A2 melanomas. J Exp Med. 1993;178(2):489-95.
 Sahin U, T?reci O, Schmitt H, Cochlovius B, Johannes T, Schmits R, Stenner F, Luo G, Schobert I, Pfreundschuh M. Human neoplasms elicit multiple specific immune responses in the autologous host. Proc Natl Acad Sci U S A. 1995;92(25):11810-3.
 Chen YT, Scanlan MJ, Sahin U, T?reci O, Gure AO, Tsang S, Williamson B, Stockert E, Pfreundschuh M, Old LJ. A testicular antigen aberrantly expressed in human cancers detected by autologous antibody screening. Proc Natl Acad Sci U S A. 1997;94(5):1914-8.
 Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987;162(1):156-9. PMID:2440339
 Schl?ter C, Duchrow M, Wohlenberg C, Becker MH, Key G, Flad HD, Gerdes J. The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle-maintaining proteins. J Cell Biol. 1993;123(3):513-22.
 Kubbutat MH, Key G, Duchrow M, Schl?ter C, Flad HD, Gerdes J. Epitope analysis of antibodies recognising the cell proliferation associated nuclear antigen previously defined by the antibody Ki-67 (Ki-67 protein). J Clin Pathol. 1994;47(6):524-8.