Biopolym. Cell. 2000; 16(5):335-338.
Structure and Function of Biopolymers
Purification of transfer factor of immune sensibilization to staphylococcal antigen substances
1Kholodna L. S., 1Lyubchenko T. A., 1Goleva O. G., 1Mikhalsky L. O.
  1. Taras Shevchenko National University of Kyiv
    64, Volodymyrska Str., Kyiv, Ukraine, 01033


The article presents the methods of obtaining, purification and fractionation of human and animal preparations of transfer factor immune sensibilization. The similarity in molecular masses and gelchromatography elution profiles has been shown. Using etectrophoresis in polyacrilamide gel it has been demonstrated that such substances are contained in fractions with the molecular mass lower than 14.4 kDa.


[1] Liubchenko TA, Holeva OH, Kholodna LS, Smirnov VV, Vershyhora AIu. The biological activity of the transfer factor induced by bacterial antigens. Mikrobiol Z. 1997;59(5):83-100.
[2] Lawrence NS. The transfer of generalized cutaneous hypersensitivity of the delayed tuberculin type in man by means of the constituents of disrupted leukocytes. J Clin Invest. 1954; 33: 951.
[3] Fudenberg HH, Pizza G. Transfer factor 1993: new frontiers. Prog Drug Res. 1994;42:309-400.
[4] Rozzo SJ, Kirkpatrick CH. Purification of transfer factors. Mol Immunol. 1992;29(2):167-82.
[5] Holieva OH, Liubchenko TA, Kholodna LS, Vershihora AIu. The isolation of a transfer factor of the delayed-hypersensitivity type to the antigenic substances of Staphylococcus aureus in guinea pigs. Fiziol Zh. 1996;42(5-6):58-65.
[6] Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227(5259):680-5.
[7] Immunological Engineering. Ed. D. Dzhirsh. M.: Meditsina 1982. 298 p.
[8] Liubchenko TA, Holeva OH, Kholodna LS, Stepanchuk VA, Vershyhora AIu. Human specific transfer factor to Staphylococcus aureus antigens. Fiziol Zh. 1997;43(3-4):25-32.
[9] Lyubchenko TA, Goleva EG, Kholodna LS, Pozur VK. Studies of mechanism of action of leukocyte dialysates on functional activity of T- and B-lymphocytes: Congr. of Mol. Medicine (Berlin, Germany, 1997). J Mol Med. 1997. 75, N 5: B59.
[10] Lyubchenko TA, Goleva OG, Kholodna LS, Pozur VK. Purification, testing and study of the mechanism of action of human and animal transfer factor to bacterial antigenic substances. 7- Ukr biochem congr. Kyiv, 1997. Vol. 1: 90-1.