Biopolym. Cell. 2000; 16(4):270-274.
Structure and Function of Biopolymers
Genotoxical effects of ribonuclease in vivo
- Taras Shevchenko National University of Kyiv
64, Volodymyrska Str., Kyiv, Ukraine, 01033 - Institute of Toxicology, Swiss Federal Institute of Technology and University of Zurich
Schwerzenbach, Switzerland, CH-8603
Abstract
The toxic and genotoxic effects of Bacillus intermedius ribonuclease at high concentrations have been established using somatic mutation and recombination test (SMART) with Drosophila melanogaster. It has been shown that ribonuclease induces gene mutations detected as single spots of mutant hairs of mwh (multiple wing hairs) phenotype on the wing blade, and leads to the morphological alterations of the Drosophila eyes, in particular to the lysis of a part of facets.
Full text: (PDF, in Russian)
References
[1]
Mikulski S, Chun H, Mittelman A, Panella T, Puccio C, Shogen K, Costanzi J. Relationship between response rate and median survival in patients with advanced nonsmall cell lung-cancer - comparison of onconase(r) with other anticancer agents. Int J Oncol. 1995;6(4):889-97.
[2]
Boque L, Wlodawer A. Of frogs and men: attempts to humanize onkonase. Absr. 4th Int. Meet. "Ribonucleases: Chemistry, Biology, Biotechnology" (Groningen, 14-18 July, 1996). Groningen, 1996. S3L5.
[3]
Saxena SK, Rybak SM, Winkler G, Meade HM, McGray P, Youle RJ, Ackerman EJ. Comparison of RNases and toxins upon injection into Xenopus oocytes. J Biol Chem. 1991;266(31):21208-14.
[4]
Saxena SK, Vasandary VM, Ardelt W, Boix E, Wu YN, Youle RJ. The mechanism of onconase toxicity and antiviral activity. Absr. 4th Int. Meet. "Ribonucleases: Chemistry, Biology, Biotechnology" (Groningen, 14-18 July, 1996). Groningen, 1996. S3P4A.
[5]
Kurinenko BM, Sobchuk LI, Kha?bullina SA, Bulgakova RSh. [Experimental research on the antitumor effectiveness of Bac. intermedius RNAse]. Eksp Onkol. 1988;10(6):54-7.
[6]
Ilinskaya O, Ivanchenko OB, Karamova NS. Bacterial ribonuclease: mutagenic effect in microbial test-systems. Mutagenesis. 1995;10(3):165-70.
[7]
Ilinskaya ON, Karamova NS, Ivanchenko OB, Kipenskaya LV. SOS-inducing ability of native and mutant microbial ribonucleases. Mutat Res. 1996;354(2):203-9.
[8]
Casarett and Doull's toxicolody: the basic science of poisons. Eds C. D. Klaassen, M. O. Admur, J. Doull. New York: McGraw-Hill, 1996. 1111 p.
[9]
Mason JM, Aaron CS, Lee WR, Smith PD, Thakar A, Valencia R, Woodruff RC, W?rgler FE, Zimmering S. A guide for performing germ cell mutagenesis assays using Drosophila melanogaster. Mutat Res. 1987;189(2):93-102.
[10]
Graf U, W?rgler FE, Katz AJ, Frei H, Juon H, Hall CB, Kale PG. Somatic mutation and recombination test in Drosophila melanogaster. Environ Mutagen. 1984;6(2):153-88.
[11]
Vogel EW. Tests for recombinagens in somatic cells of Drosophila. Mutat Res. 1992;284(1):159-75.
[12]
Makarov AA, Protasevich II, Kuznetsova NV, Fedorov BB, Korolev SV, Struminskaya NK, Bazhulina NP, Leshchinskaya IB, Hartley RW, Kirpichnikov MP, et al. Comparative study of thermostability and structure of close homologues--barnase and binase. J Biomol Struct Dyn. 1993;10(6):1047-65.
[13]
Golubenko IA, Balaban NP, Leshchinskaia IB, Volkova TI, Kle?ner GI. [Ribonuclease of Bacillus intermedius 7 P. Purification by chromatography on phosphocellulose and several characteristics of the homogeneous enzyme]. Biokhimiia. 1979;44(4):640-8.
[14]
Balaban NP, Scharipova FR, Golubenko IA, Aphanasenko GA, Volkova TI, Vershinina VI, Znamenskaya LV, Leschinskaya IB. Physico-chemical characteristics of ribonuclease Bacillus intermedius. Proc. 1st. Int. Meet. Structure and chemistry of ribonucleases. (Moscow, 28 Nov. - 2 Dec. 1988). M, 1989:349-52.
[15]
Frei H, W?rgler FE. Statistical methods to decide whether mutagenicity test data from Drosophila assays indicate a positive, negative, or inconclusive result. Mutat Res. 1988;203(4):297-308. ttp://
