Biopolym. Cell. 2000; 16(3):220-224.
http://dx.doi.org/10.7124/bc.000569
Structure and Function of Biopolymers
Methylation variability of external cytosine of CCGG-sequences in DNA of sunflower shoots and meristem callus
1Tishchenko E. N., 1Korzh L. P.
  1. Institute of Plant Physiology and Genetics, NAS of Ukraine
    31/17, Vasylkivska, Kyiv, Ukraine, 03022

Abstract

External cytosine of CCGG-sequences in DNA of undifferentiated cells of meristems primary calluses in sunflower (Helianthus annuus L.) snoots and roots is highly methylated. During the growth and differentiation of shoots of etiolated seedlings the extensive undermethylation of this palindromic site in DNA, correlating with the transcription of tissue-specific genes, has been observed. The variability of methylation of CCGG-sequences of DNA assumes that enzymatic modification of cytosine takes part in cell differentiation, perhaps, on the level of gene expression.
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References

[1] Vanyushin VF. DNA methylation in eukaryotes, a new mechanism for regulation of gene expression and cell differentiation. Usp biol khim. 1983; 24: 170-193.
[2] Doerfler W. DNA methylation and gene activity. Annu Rev Biochem. 1983;52:93-124.
[3] Buryanov YaI., Kiryanov GI. Structural and functional bases of enzymatic DNA methylation. Itogi Nauki i Tekhniki. (Ser Mol Biol; Vol. 23). M: VINITI, 1987:3-220
[4] Bianchi MW, Viotti A. DNA methylation and tissue-specific transcription of the storage protein genes of maize. Plant Mol Biol. 1988;11(2):203-14.
[5] Riggs CD, Chrispeels MJ. The expression of phytohemagglutinin genes in Phaseolus vulgaris is associated with organ-specific DNA methylation patterns. Plant Mol Biol. 1990;14(4):629-32.
[6] Kunze R, Starlinger P, Schwartz D. DNA methylation of the maize transposable element Ac interferes with its transcription. Mol Gen Genet. 1988;214(2):325–7.
[7] Wang L, Heinlein M, Kunze R. Methylation pattern of Activator transposase binding sites in maize endosperm. Plant Cell. 1996;8(4):747-58.
[8] Martin-Tanguy J, Sun LY, Burtin D, Vernoy R, Rossin N, Tepfer D. Attenuation of the Phenotype Caused by the Root-Inducing, Left-Hand, Transferred DNA and Its rolA Gene (Correlations with Changes in Polyamine Metabolism and DNA Methylation). Plant Physiol. 1996;111(1):259-267.
[9] Kiryanov GI, Kintsurashvili LN, Manamshyan TA, Noskov VA, Smirnova TA. Chromatin structure and enzymatic methylation of a-amylaze gene DNA in barley aleurone layer cells during gene exprassion induction. Biokhimiia. 1996; 61,(1):55-64.
[10] Hawkins JD . Gene Structure and Expression. Translated SB Serebryanyy. K.: Naukova Dumka, 1991. 168 p.
[11] Walbot V, Warren C. DNA methylation in the Alcohol dehydrogenase-1 gene of maize. Plant Mol Biol. 1990;15(1):121-5.
[12] Mazin AL. [Enzymatic methylation of regulatory elements in controlling the activity of genes from various groups of organisms]. Mol Biol (Mosk). 1992;26(2):244-63.
[13] Vanyushin BF, Kirnos MD. DNA methylation of higher plants and cell differentiation. Plant genome. Kiev: Naukova Dumka, 1988: 105-30.
[14] Tishchenko EN, Kuntsevich VI, Bilinskaya AT, Lobov VP. Nuclear RNA in Helianthus annuus vegetative organs. Fiziologiia rasteniy. 1996; 43(2):213-9.
[15] Draper J, Scott R, Armitage P, Walden R, eds. Plant genetic transformation and gene expression: a Laboratory Manual. Oxford: Blackwell Scientific, 1988; 366 p.
[16] Maniatis T, Fritsch EF, Sambrook J. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Lab, 1982; 545 p.
[17] Rosin A, Cedar H. DNA methylation in eukaryotic cell. Plant Mol Biol. Manual. 1988; B3: 1-28.
[18] Savel'ev SV, Ashapkin VV, Vanyushin BF. Ribosomal DNA of hexaploid wheat: methylation pattern and replication timing in developing seedlings. Mol Biol (Mosk). 1990; 24(4):1042-56.
[19] Loschiavo F, Pitto L, Giuliano G, Torti G, Nuti-Ronchi V, Marazziti D, Vergara R, Orselli S, Terzi M. DNA methylation of embryogenic carrot cell cultures and its variations as caused by mutation, differentiation, hormones and hypomethylating drugs. Theor Appl Genet. 1989;77(3):325-31.
[20] Morrish FM, Vasil IK. DNA Methylation and Embryogenic Competence in Leaves and Callus of Napiergrass (Pennisetum purpureum Schum.). Plant Physiol. 1989;90(1):37-40.
[21] Kunakh VA. Genome variability in plant somatic cells. 4. Variability in the process of dedifferentiation and callus formation in vitro. Biopolym Cell. 1998; 14(4):298-319.