Biopolym. Cell. 1999; 15(6):557-567.
http://dx.doi.org/10.7124/bc.00054E
Methods
Interaction of nucleic acids with cyanine dyes. 15. Spectral-fluorescence properties of novel benzothiazolmethilene-2,5-dimethylpyndine homodi- and homotrimere cyanine. dyes and their complexes with nucleic acids
1Kocheshev I. O., 1Kovalska V. B., 1Yarmoluk S. M., 2Akerman B., 1Matsuka G. Kh.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Chalmers University of Technology
    SE-412 96 Gothenburg, Sweden

Abstract

Spectral properties of novel homodi- and homotrimere cyanine dyes and their complexes with nucleic acids have been investigated. The dyes studied have shown the increasing of fluorescence intensity up to 1200 and 800 times under the binding with the DNA and with the RNA respectively which make them promising as fluorescent probes for nucleic acids detection. The nature and character of aggregation of free and complexed dyes have been studied. Possible aggregate structures have been proposed.
Full text: (PDF, in Ukrainian)

References

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