Biopolym. Cell. 1999; 15(6):516-521.
Genome and Its Regulation
Gene cpn60-1 coding one of homologous chaperonins in Rhizobiuin leguminosarum is essential for cell life
1Yerko V. N., 2Lund P. A.
- Institute of Plant Physiology and Genetics, NAS of Ukraine
31/17, Vasylkivska, Kyiv, Ukraine, 03022 - School of Biosciences, The University of Birmingham
Birmingham, B15 2TT, UK
Abstract
Using the method of site specific mutagenesis we have tried to knock-out one of three homologous genes for chaperonins synthesis in Rhizobium leguminosarum bv. viceae. It has been shown that cpn60-1 gene cannot be destroyed due to its essentiality for cell life.
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