Biopolym. Cell. 1999; 15(6):516-521.
http://dx.doi.org/10.7124/bc.000548
Genome and Its Regulation
Gene cpn60-1 coding one of homologous chaperonins in Rhizobiuin leguminosarum is essential for cell life
1Yerko V. N., 2Lund P. A.
  1. Institute of Plant Physiology and Genetics, NAS of Ukraine
    31/17, Vasylkivska, Kyiv, Ukraine, 03022
  2. School of Biosciences, The University of Birmingham
    Birmingham, B15 2TT, UK

Abstract

Using the method of site specific mutagenesis we have tried to knock-out one of three homologous genes for chaperonins synthesis in Rhizobium leguminosarum bv. viceae. It has been shown that cpn60-1 gene cannot be destroyed due to its essentiality for cell life.
Full text: (PDF, in Russian)

References

[1] Anfinsen CB. Principles that govern the folding of protein chains. Science. 1973;181(4096):223-30.
[2] Ellis J. Proteins as molecular chaperones. Nature. 1987 Jul 30-Aug 5;328(6129):378-9.
[3] Zeilstra-Ryalls J, Fayet O, Georgopoulos C. The universally conserved GroE (Hsp60) chaperonins. Annu Rev Microbiol. 1991;45:301-25.
[4] Boorstein WR, Ziegelhoffer T, Craig EA. Molecular evolution of the HSP70 multigene family. J Mol Evol. 1994;38(1):1-17.
[5] Fayet O, Ziegelhoffer T, Georgopoulos C. The groES and groEL heat shock gene products of Escherichia coli are essential for bacterial growth at all temperatures. J Bacteriol. 1989;171(3):1379-85.
[6] Paek KH, Walker GC. Escherichia coli dnaK null mutants are inviable at high temperature. J Bacteriol. 1987;169(1):283-90.
[7] Rusanganwa E, Gupta RS. Cloning and characterization of multiple groEL chaperonin-encoding genes in Rhizobium meliloti. Gene. 1993;126(1):67-75.
[8] Babst M, Hennecke H, Fischer HM. Two different mechanisms are involved in the heat-shock regulation of chaperonin gene expression in Bradyrhizobium japonicum. Mol Microbiol. 1996;19(4):827-39.
[9] Wallington EJ, Lund PA. Rhizobium leguminosarum contains multiple chaperonin (cpn60) genes. Microbiology. 1994;140 ( Pt 1):113-22.
[10] Ogawa J, Long SR. The Rhizobium meliloti groELc locus is required for regulation of early nod genes by the transcription activator NodD. Genes Dev. 1995;9(6):714-29.
[11] Simon R., Priefer U., Puhler A. A broad host range mobilization system for in vivo genetic engineering: Transposon mutagenesis in gram negative bacteria. Bio/Technology, 1983; 1(9):784-791.
[12] Quandt J, Hynes MF. Versatile suicide vectors which allow direct selection for gene replacement in gram-negative bacteria. Gene. 1993;127(1):15-21.
[13] Maniatis T., Pritsch E.F., Sambrook J. Molecular Cloning: J Laboratory Manual, New York: Cold Spring Harbor Lab. press 1989; p. 545.
[14] Beringer J.E., Beynon J.L., Buchanan-Wollaston A.V., Johnston A.W.B. Transfer of the drug-resistance transposon Tn5 to Rhizobium. Nature, 1978; 276 (5688):633-634.
[15] Genetic breeding techniques nodule bacteria (guidelines). L.: ARRIAM, 1984.
[16] Ausubef F.M. Current Protocols In Molecular Biology, New York: Willey Intersci 1987; p. 320.
[17] Birnboim HC, Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979;7(6):1513-23.