Shikonin accumulation and cytological peculiarities of highly productive Arnebia euchroma cell strain upon surface and submerged maintenance

Poronnyk, O. A.; Kunakh, V. A.; Adonin, V. I.

doi:10.7124/bc.000546

Biopolym. Cell. 1999; 15(6):501-509.

http://dx.doi.org/10.7124/bc.000546

Cell Biology

Shikonin accumulation and cytological peculiarities of highly productive Arnebia euchroma cell strain upon surface and submerged maintenance

1Poronnyk O. A., 1Kunakh V. A., 1Adonin V. I.

Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

A. euchroma novel cell strain AE-3, accumulating about 80 and 100 mg/1 shikonin per day upon maintenance in suspension culture and on agarized medium, respectively, has been studied. The study on cell and nucleus size, their morphology, evaluation of mitotic activity, chromosome number, level and types of their aberration failed to demonstrate any essential difference under the various conditions of maintenance. The strain appears to be mixoploid showing chromosome variation range from 10 to 130 chromosomes with modal classes formed by cells carrying 30 to 37 chromosomes and characterized by low level of antiphase chromosome aberration (about 6 %). Comparison of various A. euchroma cell lines has not revealed any relationship between their productivity and cytological and cytogenetic peculiarities.

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