Biopolym. Cell. 1999; 15(6):496-500.
http://dx.doi.org/10.7124/bc.000545
Cell Biology
Reduction of plastid protein synthesis apparatus is a base of stable chlorophyll deficiency induced by antibiotics in plants
1Zubko M. K.
  1. Institute of Cell Biology and Genetic Engineering, NAS of Ukraine
    148, Akademika Zabolotnogo Str., Kyiv, Ukraine, 03680

Abstract

A molecular base for the stable chlorophyll deficiency induced with high frequency by antibiotics spectinomycin and streptomycin in cruciferous plants has been studied. It has been shown that the most probable cause of chlorophyll deficiency is knockouting plastid protein biosynthesis due to the reduction of plastid protein synthesis apparatus, in particular, ribosomal RNA as the most important component. This phenomenon is proposed as a model for studying biosynthetic functions of plastids.
Full text: (PDF, in Russian)

References

[1] Kirk J.T.O., Tilney-Bassett R.A.E. 1978. The Plastids. Amsterdam: Elsevier
[2] Ellis R.J. Inhibitors for studying chloroplast transcription and translation in vivo II Methods in Chloroplast Molecular Biology. Eds Edelman et al. Amsterdam: Elsevier 1982; 559-564.
[3] Borner T., Sears B.B. Plastome mutants. Plant Molecular Biology Reporter, 1986; 4 (2):69-92.
[4] Feierabend J., Berberich T. Heat-induced ribosome-deficiency of plastids mechanisms and applications. The Translational Apparatus of Photosynthetic Organelles, Eds R. Mache. Berlin, Heidelberg: Springer 1991;215-227.
[5] Zubko M. K. Stable chlorophyll induced in cruciferous plants with antibiotics. Fiziologiya i biokhimiya kul'turn, rasteniy. 1999; 6:477.
[6] Murashige T, Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant. 1962;15(3):473–97.
[7] Poulson, R. Isolation, purification and fractionation of RNA. The Ribonucleic Acids, Eds Stewart P., Letham D.-Berlin: Springer 1973 pp. 243-261.
[8] Day A., Ellis T.H.N. Deleted forms of plastid DNA in albino plants from cereal anther culture. Current Genetics, 1985; 9(8):671-678.
[9] Sugiura M., Shinozaki K., Zaita N., Kusuda M., Kumano M. Clone bank of the tobacco (Nicotiana tabacum) chloroplast genome as a set of overlapping restriction endonuclease fragments: Mapping of eleven ribosomal protein genes. Plant Science, 1986; 44(3):211-217.
[10] Goldsbrough PB, Cullis CA. Characterisation of the genes for ribosomal RNA in flax. Nucleic Acids Res. 1981;9(6):1301-9.
[11] Sager R. Streptomycin as a mutagen for nonchromosomal genes. Proc Natl Acad Sci U S A. 1962;48:2018-26.
[12] Sarma N.P., Patnaik A. Streptomycin induced nuclear & cytoplasmic chloroplast mutations in rice. Indian Journal of Experimental Biology, 1982; 20 (2):177-178.
[13] Hess WR, Muller A, Nagy F, Borner T. Ribosome-deficient plastids affect transcription of light-induced nuclear genes: genetic evidence for a plastid-derived signal. Mol Gen Genet. 1994;242(3):305-12.
[14] Svab Z, Maliga P. Nicotiana tabacum mutants with chloroplast encoded streptomycin resistance and pigment deficiency. Theor Appl Genet. 1986;72(5):637-43.