Biopolym. Cell. 1999; 15(5):367-373.
Structure and Function of Biopolymers
Synthesis and study of antisense oligonuclcolides modified with imidazophenazine nucleosides
1Makitruk V. L., 1Shalamay A. S., 2Dubey I. Y., 2Fedoryak D. M.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine
    1, Murmans'ka Str., Kyiv, Ukraine, 02094

Abstract

Oligonucleotides containing imidazophenazine nucleosides at 3'-, 5'-and internal positions, which are complementary to certain regions of ribosomal operon DNA and I6S rRNA of mycoplasmas and E. coli, have been synthesized by H-phosphonate solid phase method. It has been shown that nucleosides of imidazol [4,5-b]phenazine and its 2-methyl analog as efficient intercalating agents strongly affect the stability of complementary complexes increasing the antisense activity of modified otigonucleotide sequence.

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