Biopolym. Cell. 1999; 15(4):289-291.
Structure and Function of Biopolymers
The influence of low doses of radiation on the content of superoxide dismutase RNA in different tissues of mice
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
The content of mRNA coding copper-zinc superoxide dismutase (CuZn-SOD) was studied in cells of different tissues of mice. With the help of DNA-RNA hybridization it was found that long effect of low doses of radiation causes the inhibition of CuZn-SOD transcription In spleen and testicle and stimulates the gene expression of this enzyme in liver and brain of irradiated animals. The question about possible participation of CuZn-SOD in rod lores istance of mammals cells is discussed.
 Gusev VA, Brusov OS, Panchenko LF. Superoxide dismutase--radiobiologic significance and possibilities (review). Vopr Med Khim. 1980;26(3):291-301.
 Peng TX, Moya A, Ayala FJ. Irradiation-resistance conferred by superoxide dismutase: possible adaptive role of a natural polymorphism in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1986;83(3):684-7.
 Goncharenko EN, Gudz' TI, Peshkova EG. Superoxide dismutase activity in animals with radiation sickness. Nauchnye Doki Vyss Shkoly Biol Nauki. 1980;(7):34-7.
 Lipinski S, Lipecka K, Doniec J, Kanski M. Effect of ionizing radiation on the activity of superoxide dismutase determined by the chemoluminescence method. Radiobiologiia. 1976;16(5):665-70.
 Barabo? VA, Oliinik SA, Khmielievskyi IuV. Status of the antioxidant system at low doses and low intensities of ionizing radiation. Ukr Biokhim Zh. 1994;66(4):3-18
 Yamaoka K, Edamatsu R, Mori A. Time dependent changes in SOD activities and lipid peroxides levels in organs of rats after low dose X:Ray irradiation. J. Radiat. Res., 1991;32 (1):73.
 Gatsko GG, Mazhul' LM, Shablinskaia OV, Volykhina VE. The effect of ionizing radiation on lipid peroxidation in rat blood. Radiobiologiia. 1990;30(3):413-5.
 Tanaka H, Iwasaki S, Suzuki H. Biochemical analyses ofcerebrum of fetal rats X-irradiated in utero. Content and composition of DNA, superoxide dismutase and lipid peroxide. Radiat. Risks Developing Nervous Syst. Int. Symp., Stuttgart; New York 1986 pp. 231-242.
 Gatsko GG, Mazhul' LM, Volykhina VYe. Et al. Effect of high radiation background on lipid peroxidation in the blood of experimental animals material I scientific-practical. conf. Minsk. 1990; 203-208.
 Raha S, Merante F, Proteau G, Reed JK. Simultaneous isolation of total cellular RNA and DNA from tissue culture cells using phenol and lithium chloride. Genet Anal Tech Appl. 1990;7(7):173-7.
 Kovalev V. A., Troyanovskiy B. M Cloning of Cu/Zn-dependent superoxide dismutase in human expression vector plasmids. New directions for biotechnology: Tez. dokl. Vsesoyuz. konf. M., 1988; 54.
 Braga EA, Avdonina TA, Zhurkin VB, Nosikov VV. Structural organization of rat ribosomal RNA genes: interspersed sequences and their putative role in the alignment of nucleosomes. Gene. 1985;36(3):249-62.
 Feinberg AP, Vogelstein B. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum. Anal Biochem. 1984;137(1):266-7.
 Hartz JW, Funakoshi S, Deutsch HF. The levels of superoxide dismutase and catalase in human tissues as determined immunochemically. Clin Chim Acta. 1973;46(2):125-32.
 Otero G, Avila MA, Emfietzoglou D, Clerch LB, Massaro D, Notario V. Increased manganese superoxide dismutase activity, protein, and mRNA levels and concurrent induction of tumor necrosis factor alpha in radiation-initiated Syrian hamster cells. Mol Carcinog. 1996;17(4):175-80.
 Zhong W, Oberley LW, Oberley TD, Yan T, Domann FE, St Clair DK. Inhibition of cell growth and sensitization to oxidative damage by overexpression of manganese superoxide dismutase in rat glioma cells. Cell Growth Differ. 1996;7(9):1175-86.
 Epperly M, Bray J, Kraeger S, Zwacka R, Engelhardt J, Travis E, Greenberger J. Prevention of late effects of irradiation lung damage by manganese superoxide dismutase gene therapy. Gene Ther. 1998;5(2):196-208.
 Sun J, Chen Y, Li M, Ge Z. Role of antioxidant enzymes on ionizing radiation resistance. Free Radic Biol Med. 1998;24(4):586-93.