Biopolym. Cell. 1999; 15(4):283-288.
http://dx.doi.org/10.7124/bc.000526
Structure and Function of Biopolymers
Hybridomas producing the monoclonal antibodies to the cotton membrane proteins
1Khashimova Z. S., 1Mangutova Ju. S., 1Suslo M. E., 1Leontjev V. B.
  1. A. S. Sadykov Institute of Bioorganic Chemistry
    83, Mirzo-Ulugbek Str, Tashkent, Uzbekistan, 100125

Abstract

Monoclonal antibodies (mcAB) to membrane antigens were generated by immunization of BALB/c mices with total plasma membrane proteins from cotton seedlings. 12 stable hybridoma clones secreting mcAB directed to the components of the total membrane proteins were selected; reactivity of these mcAB was determined using ELISA methods. McAB to surface epitopes of isolated cotton protoplasts, membrane and membrane-bound proteins, cell-wall protein (extensin), some groups of lectin-like proteins from cotton seeds have been described, their immunochemical cross-reactivity was discussed. McAB to cotton plasma-membrane proteins can be used as biochemical markers and for fractionation and membrane proteins functional activity study.
Full text: (PDF, in Russian)

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