Biopolym. Cell. 1999; 15(3):237-240.
http://dx.doi.org/10.7124/bc.00051F
Viruses and Cell
Callus induction in mature corn embryos
1Checheneva T. N., 1Luchko L. V., 1Dykun M. O.
  1. Institute of Plant Physiology and Genetics, NAS of Ukraine
    31/17, Vasylkivska, Kyiv, Ukraine, 03022

Abstract

Ways of increasing of totipotent callus induction for mature embryo corn inbred lines were investigated. Using mature embryos from mature corn grains of somaclonal varients of tested lines led to the increasing of callus formation, level. Best results were obtained when casein hydrolysate was added to callus induction medium and 6-benzylaminopurine was added to regeneration- medium.
Full text: (PDF, in Russian)

References

[1] Tomes DT, Smith OS. The effect of parental genotype on initiation of embryogenic callus from elite maize (Zea mays L.) germplasm. Theor Appl Genet. 1985;70(5):505-9.
[2] Checheneva TN, Morgun VV, Ruban TA. Regeneration of plant from different types of callus tissue of inbred lines and maize hybrids. Doklady Akad Nauk Ukr SSR. Ser B. 1988;(11):80-3.
[3] Checheneva T. YA. Trukhamov V. L. offshoot morphogenesis and somatic embryogenesis in tissue culture of maize. Experimental plant genetics to accelerate the breeding process. Kiev: Naukova Dumka, 1989:120-128.
[4] Hodges TK, Kamo KK, Imbrie CW, Becwar MR. Genotype Specificity of Somatic Embryogenesis and Regeneration in Maize. Nature Biotechnology. 1986. 4, N 3: 219—223.
[5] Chernysheva VG, Dolgikh YUI, Shamina ZB, Butenko RG. Influence of genetic characteristics of the original plants on morpho genetic potential of maize callus cells. Dokl. AN SSSR. 1938; 300(1):227—229.
[6] Checheneva TYA, Trukhanov VA. genetically caused kallusobrazovaniya and regenerative capacity in maize. Tsitol Genet. 1994; 28(5):46-50.
[7] Lu C, Vasil V, Vasil IK. Improved efficiency of somatic embryogenesis and plant regeneration in tissue cultures of maize (Zea mays L.). Theor Appl Genet. 1983;66(3-4):285-9.
[8] Duncan DR, Singletary GW, Below FE, Widholm JM. Increased induction of regenerable callus cultures from cultured kernels of the maize inbred FR27rhm. Plant Cell Rep. 1989;8(6):350-3.
[9] Green CE, Philips RL. Plant Regeneration from Tissue Cultures of Maize1. Grop Sci. 1975;15(5):417—421.
[10] Vasil IK. Developing cell and tissue culture systems for the improvement cereal and grass crops. J Plant Physiol. 1987; 128(3):193—218.
[11] Checheneva TN, Truchanov VA. The increase of regenerative ability in maize inbred lines in vitro. Tsitol Genet 1997; 31(2):36-40.
[12] Dospekhov B. A. Methodology field experience. Moscow: Kolos, 1973. 335.
[13] Kunakh VA, Checheneva TN, Morgun VV. Production of callus tissues from maize plants of various genotypes. Fiziologiia rasteniy. 1980; 27(2):399-403.