Biopolym. Cell. 1999; 15(1):67-74.
Viruses and Cell
New packaging cells based on the: adapted variant of the Rous sarcoma virus
1Kaida I. V., 2Serov S. M., 1Michailik A. A., 1Boltovets P. M., 1Kashuba V. I., 1Rynditch A. V.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
    32, Vavilova Str., Moscow, Russian Federation, 119991


We have constructed a new transformation-defective mutant of duck-adapted variant of Rouse sarcoma virus Prague strains subgroup C (td daPr-RSV-C) – based packaging cell line. F18/pJD which produces replication-defective vector with a liter 106 CfU/ml. Deletion of cis-acting sequences of packaging region and E (direct repeat flanking src) in the adapted variant of retrovirus allowed us to obtaine a pool of retrovirus vector without helper virus sequences.


[1] Kaida IV, Rynditch AV. Usage of retrovirus vectors for gene transfer into birds. Biopolym. Cell. 1997;13(1):5-13.
[2] Kaida IV, Tsyba LA, Boltovets PN, Kashuba VI. A new retroviral vector system based on the duck-adapted variant of Pr-RSV-C with the extended host ranger. Biopolym. Cell. 1997;13(5):408-415
[3] Boerkoel CF1, Federspiel MJ, Salter DW, Payne W, Crittenden LB, Kung HJ, Hughes SH. A new defective retroviral vector system based on the Bryan strain of Rous sarcoma virus. Virology. 1993;195(2):669-79.
[4] Savatier P1, Bagnis C, Thoraval P, Poncet D, Belakebi M, Mallet F, Legras C, Cosset FL, Thomas JL, Chebloune Y, et al. Generation of a helper cell line for packaging avian leukosis virus-based vectors. J Virol. 1989;63(2):513-22.
[5] Cosset FL, Legras C, Chebloune Y, Savatier P, Thoraval P, Thomas JL, Samarut J, Nigon VM, Verdier G. A new avian leukosis virus-based packaging cell line that uses two separate transcomplementing helper genomes. J Virol. 1990;64(3):1070-8.
[6] Cosset FL, Ronfort C, Molina RM, Flamant F, Drynda A, Benchaibi M, Valsesia S, Nigon VM, Verdier G. Packaging cells for avian leukosis virus-based vectors with various host ranges. J Virol. 1992;66(9):5671-6.
[7] Dougherty JP, Wisniewski R, Yang SL, Rhode BW, Temin HM. New retrovirus helper cells with almost no nucleotide sequence homology to retrovirus vectors. J Virol. 1989;63(7):3209-12.
[8] Katz RA, Terry RW, Skalka AM. A conserved cis-acting sequence in the 5' leader of avian sarcoma virus RNA is required for packaging. J Virol. 1986;59(1):163-7.
[9] Sorge J, Ricci W, Hughes SH. cis-Acting RNA packaging locus in the 115-nucleotide direct repeat of Rous sarcoma virus. J Virol. 1983;48(3):667-75.
[10] Donze O, Spahr PF. Role of the open reading frames of Rous sarcoma virus leader RNA in translation and genome packaging. EMBO J. 1992;11(10):3747-57.
[11] Anderson DJ, Lee P, Levine KL, Sang JS, Shah SA, Yang OO, Shank PR, Linial ML. Molecular cloning and characterization of the RNA packaging-defective retrovirus SE21Q1b. J Virol. 1992;66(1):204-16.
[12] Aronoff R, Linial M. Specificity of retroviral RNA packaging. J Virol. 1991;65(1):71-80.
[13] Aronoff R, Hajjar AM, Linial ML. Avian retroviral RNA encapsidation: reexamination of functional 5' RNA sequences and the role of nucleocapsid Cys-His motifs. J Virol. 1993;67(1):178-88.
[14] Sakalian M, Wills JW, Vogt VM. Efficiency and selectivity of RNA packaging by Rous sarcoma virus Gag deletion mutants. J Virol. 1994;68(9):5969-81.
[15] Hu S, Bruszewski J, Nicolson M, Tseng J, Hsu RY, Bosselman R. Generation of competent virus in the REV helper cell line C3. Virology. 1987;159(2):446-9.
[16] Girod A, Drynda A, Cosset FL, Verdier G, Ronfort C. Homologous and nonhomologous retroviral recombinations are both involved in the transfer by infectious particles of defective avian leukosis virus-derived transcomplementing genomes. J Virol. 1996;70(8):5651-7.
[17] Ronfort C, Girod A, Cosset FL, Legras C, Nigon VM, Chebloune Y, Verdier G. Defective retroviral endogenous RNA is efficiently transmitted by infectious particles produced on an avian retroviral vector packaging cell line. Virology. 1995;207(1):271-5.
[18] Donahue RE, Kessler SW, Bodine D, McDonagh K, Dunbar C, Goodman S, Agricola B, Byrne E, Raffeld M, Moen R, et al. Helper virus induced T cell lymphoma in nonhuman primates after retroviral mediated gene transfer. J Exp Med. 1992;176(4):1125-35.
[19] Norton PA, Coffin JM. Characterization of Rous sarcoma virus sequences essential for viral gene expression. J Virol. 1987;61(4):1171-9.
[20] Molina RM, Chebloune Y, Verdier G, Legras C. Influence of expression and cis-acting sequences from avian leukosis viruses (ALVs) on stability of (ALV)-based retrovirus vectors. C R Acad Sci III. 1995;318(5):541-51.
[21] Kashuba VI, Kavsan VM, Ryndich AV, Lazurkevich ZV, Zubak SV, Popov SV, Dostalova V, Glozhanek I. Complete nucleotide sequence of Rous sarcoma virus variants adapted to duck cells Mol Biol (Mosk). 1993;27(2):436-50.
[22] Pogrebnoi PV, Serov SM, Klenchin VA, Luk'ianov AV, Garmanchuk LV, Tarnavskii DV, Markeeva NV, Bykorez AI. Expression of the gene for transforming growth factor type alpha, transferred into A431 cells by a recombinant retroviral vector Mol Biol (Mosk). 1993;27(4):833-8.
[23] Moscovici C, Moscovici MG, Jimenez H, Lai MM, Hayman MJ, Vogt PK. Continuous tissue culture cell lines derived from chemically induced tumors of Japanese quail. Cell. 1977;11(1):95-103.
[24] Clark DP, Dougherty RM. Detection of avian oncovirus group-specific antigens by the enzyme-linked immunosorbent assay. J Gen Virol. 1980;47(2):283-91.
[25] Tereba A, Murti KG. A very sensitive biochemical assay for detecting and quantitating avian oncornaviruses. Virology. 1977;80(1):166-76.
[26] Svoboda J, Machala O, Deozanek T. Rescue of Rous sarcoma virus in mixed cultures of virogenic mammalian and chicken cells, treated and untreated with Sendai virus and detected by focus assay. J Gen Virol. 1968;2(3):461-4.
[27] Hajkova V, Kristek J, Geryk J, Svoboda J. Characterization of a mutant of the Prague strain of Rous sarcoma virus inducing fusiform transformation of avian fibroblasts in vitro. Folia Biol (Praha). 1982;28(3):160-76.
[28] Kashuba VI, Zubak SV, Lazurkevich ZV, Ryndich AV, Kavsan VM. Structure of a new transformation-defective mutant of Rous sarcoma virus. Dokl Akad Nauk SSSR. 1989;304(1):206-8.
[29] Kashuba VI, Zubak SV, Rynditch AV, Kavsan VM, Hlozanek I, Svoboda J. The nucleotide sequence of the region of src gene deletion in transformation-defective Rous sarcoma virus adapted to semi-permissive host cells. Nucleic Acids Res. 1989;17(8):3294.
[30] Mann R, Mulligan RC, Baltimore D. Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus. Cell. 1983;33(1):153-9.
[31] Knight JB, Si ZH, Stoltzfus CM. A base-paired structure in the avian sarcoma virus 5' leader is required for efficient encapsidation of RNA. J Virol. 1994;68(7):4493-502.