Cloning and bacterial expression of the cytokine-like noncatalytic domain of bovine tyrosyl-tRNA synthetase

Dubrovsky, A. L.; Savinskaya, L. A.; Kornelyuk, A. I.

doi:10.7124/bc.0004EA

Biopolym. Cell. 1998; 14(5):449-452.

http://dx.doi.org/10.7124/bc.0004EA

Structure and Function of Biopolymers

Cloning and bacterial expression of the cytokine-like noncatalytic domain of bovine tyrosyl-tRNA synthetase

1Dubrovsky A. L., 1Savinskaya L. A., 1Kornelyuk A. I.

Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Cytokine-like noncatalytical C-terminal domain of mammalian tyrosyl-tRNA synthetase (D322-S528) has been cloned and expressed in bacterial expression system using pET15b vector. Recombinant protein has been isolated by metal-chelating chromatography on the Ni-NTA agarose into a homogenous state according to gel-electrophoresis data.

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