Biopolym. Cell. 1998; 14(3):238-241.
Gene-Engineering Biotechnology
Isolation and restriction analysis of two plasmids from Streptomyces globisporus 1912
- D. K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154, Academika Zabolotnogo Str., Kyiv, Ukraine, 03680 - Georg-August-Universitat Gottingen, Institute for Organic and Biomolecular Chemistry
Tammannstr. 2, 37077 Gottingen, Germany
Abstract
Two plasmids pSG1912-1 (10,3 kb) and pSG1912-2 (22,4 kb) were isolated from mycelium of three Streptomyces globisporus strains differing in the level of sporulation and landomycin E production. The number of restriction sites for endonucleases Bglll, Kpnl, PviJI and Sad was determined in both plasmid molecules.
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