Biopolym. Cell. 1998; 14(2):117-126.
http://dx.doi.org/10.7124/bc.0004C3
Structure and Function of Biopolymers
1H-NMR investigation of coraplexation of acridine dye proflavine with deoxytetraribonucleoside triphosphate 5'-d(TpGpCpA) in aqueous solution
1Eaton R. A., 1Veselkov D. A., 2Djimant L. N., 2Baranovsky S. F., 2Osetrov S. G., 1Davies D. B., 2Veselkov A. N.
  1. Birkbeck, University of London
    Malet Str., Bloomsbury, London WC1E 7HX, UK
  2. Sevastopol National Technical University
    33, Universytetska Str., Sevastopol, Ukraine, 99053

Abstract

The interaction of acridine dye proflavine with self-complementary deoxytetraribonucleoside triphosphate 5'-d(TpGpCpA) in aqueous salt solution has been studied by one- and two- dimentional 500 MHz 1H-NMR spectroscopy. Concentration and temperature dependences of proton chemical shifts of the interacting molecules have been measured. Different schemes of complexation of proflavine with the tetranucleotide have been analysed and the equilibrium constants, enthalpies ΔH, entropies ΔS of different reactions leading to the formation of 1:1, 1:2, 2:1, 2:2 complexes have been determined. The specific features of the dynamic equilibrium of different complexes as a function of the drug-tetranucleotide ratio have been examined. It is concluded that proflavine intercalates preferentially to pyrimidine-purine TG- and CA-sites of the tetranucleotide sequence. Comparative analysis of the distinctive features of the intercalative binding of proflavine and that of phenan-tridinium dye ethidium bromide studied earlier has shown that intensity of selective interaction of aromatic ligands with pyrimidine-purine sequence depends on the base content and type of the bases flanking the binding sites. The most favourable structures 1:2 and 2:2 proflavine-tetranucleotide complexes have been constructed using calculated values of induced chemical shift of dye protons and 2D-NOE spectra.
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