Biopolym. Cell. 1998; 14(1):54-61.
Structure and Function of Biopolymers
Stabilization of duplex and triplex complexes of oligothymidylate by covalently linked imidazophenazine glycoside
1Zozulya V. N., 1Blagoi Yu. P., 2Dubey I. Y., 2Fedoryak O. D., 1Shcherbakova A. S., 2Fedoryak D. M.
  1. B. I. Verkin Institute for Low Temperature Physics and Engineering, NAS of Ukraine
    47, Prospekt Lenina, Kharkiv, Ukraine, 61103
  2. Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine
    1, Murmans'ka Str., Kyiv, Ukraine, 02094


Decathymidylate containing nucleoside derivative of imidazophenazine (Pzn) at the 3'-end was synthesized. The effect of dye covalent attachment on the formation of complementary complexes of (dT)10, namely duplex with (dA)15 and triplex with (dA)15 and poly(dA)·poly(dT), was studied in buffer solutions of neutral pH at ionic strength μ–0,1 and 1 M. Thermal denaturation method using absorption and fluorescence spectroscopy was employed. It has been shown that Pzn residue strongly stabilized duplex and triplex complexes by dye chromophore intercalation into dA and dA·dT sequences. Melting point of complexes increased for 10–12 °C for duplex and 15–20 °C for triplex structures. Stabilizing effect of neutral imidazophenazine was comparable to that of cationic intercalating dyes linked to oligonucleotides via polymethylene linker.


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