Biopolym. Cell. 1998; 14(1):23-28.
Structure and Function of Biopolymers
Biosensors based on cholinesterases for the analysis of pesticides, heavy metal ions and hypochlorite species
1Soldatkin A. P.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

The enzyme biosensors based on cholinesterases and electrochemical transducers were developed for the analysis of pesticides, heavy metal ions and hypochlorite species in water solutions. Bioselective membranes on the transducers surface were formed by cross-linking of cholinesterases with bovine serum, albumin in saturated glu-taraldchyde vapour. Working characteristics of the biosensors developed were studied and optimized.

References

[1] Handbook of analytical chemistry. Ed. L. Meites. New York etc.: McGraw-Hill Book Company, 1963.
[2] Baylocq-Ferrier D, Baillet-Guffroy A, Pellerin F. Methodes generales d'analyse quantitative. Titrage par iodomctrie. Traite: Analyse et Caracterisation. Ed. C. Genty-Paris: Techniques de ringenieur, 1995: 330-1-330-8.
[3] Shul’ga AA, Netchiporouk LI, Sandrovsky AK, Abalov AA, Frolov OS, Kononenko YG, et al. Operation of an ISFET with non-insulated substrate directly exposed to the solution. Sens Actuators B Chem. 1996;30(2):101–5.
[4] Zhylyak GA, Dzyadevich SV, Korpan YI, Soldatkin AP, El’skaya AV. Application of urease conductometric biosensor for heavy-metal ion determination. Sens Actuators B Chem. 1995;24(1-3):145–8.
[5] Tran-Minh C. Immobilised enzyme probes for determining inhibitors. Ion-Selec. Electrode Rev. 1985. 7: 41-75.
[6] Nyamsi Hendji AM, Jaffrezic-Renault N, Martelet C, Shul’ga AA, Dzydevich SV, Soldatkin AP, et al. Enzyme biosensor based on a micromachined interdigitated conductometric transducer: application to the detection of urea, glucose, acetyl- andbutyrylcholine chlordes. Sens Actuators B Chem. 1994;21(2):123–9.
[7] Hendji AMN, Jaffrezic-Renault N, Martelet C, Clechet P, Shlu’ga AA, Strikha VI, et al. Sensitive detection of pesticides using a differential ISFET-based system with immobilized cholinesterases. Anal Chim Acta. 1993;281(1):3–11.
[8] Dzydevich SV, Shu?ga AA, Soldatkin AP, Hendji AMN, Jaffrezic-Renault N, Martelet C. Conductometric biosensors based on cholinesterases for sensitive detection of pesticides. Electroanalysis. 1994;6(9):752–8.
[9] Korpan YaI, Soldatkin AP, Gonchar MV, Sibirny AA, Gibson TD, El'skaya AV. A novel enzyme biosensor specific for formaldehyde based on ph-sensitive field effect transistors. J Chem Technol Biotechnol. 1997; 68(2):209-13.
[10] Albrich JM, Hurst JK. Oxidative inactivation of Escherichia coli by hypochlorous acid. Rates and differentiation of respiratory from other reaction sites. FEBS Lett. 1982;144(1):157-61.