Biopolym. Cell. 1997; 13(6):460-463.
http://dx.doi.org/10.7124/bc.0004A7
Structure and Function of Biopolymers
DNA in bands of poiytene chromosomes is not under lorsional tension
1Gruzdev A. D., 2Lezzi M.
  1. Institute of Cytology and Genetics, Siberian Branch of the RAS
    10, Akademika Lavrentieva Ave., Novosibirsk, Russian Federation, 630090
  2. Institute of Cell Biology Swiss Federal Institute of Technology Zurich
    Building HPM, Schafmattstrasse 18, CH-8093 Zurich, Swiss

Abstract

Topological state of DNA in bands of poiytene chromosomes of Camptochironomus tentans was investigated under confocal laser scanning fluorescent microscope by microfluorometric method. It was shown that the bands contain topologically closed, negatively supercoiled domains of DNA in which the value of the relative twist difference is very low and does not exceed 0.007.
Full text: (PDF, in Russian)

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