Biopolym. Cell. 1997; 13(5):419-421.
http://dx.doi.org/10.7124/bc.0004A1
Methods
Interaction of cyanine dyes with nucleic acids. 3. The of new cyanine dyes Cyan 13 and Cyan 40 for detection of nucleic acids in agarose gel
1Yarmoluk S. M., 2Dubey I. Ya.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine
    1, Murmans'ka Str., Kyiv, Ukraine, 02094

Abstract

Detection of double-stranded DNA (dsDNA) and single-strended DNA (ssDNA) and RNA with two new cyanine dyes Cyan 13 and Cyan 40 is reported. Cyan 13 and Cyan. 40 bind to nucleic acids to form a stable fluorescent complexes and can be used for the detection of DMA and RNA samples: separated by gel electrophoresis. Sensitivity of detection is comparable to tfat for ethidium bromide (EtBr), a common nucleic add staining dye.
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References

[1] Birnboim HC. A rapid alkaline extraction method for the isolation of plasmid DNA. Methods Enzymol. 1983;100:243-55.
[2] Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987;162(1):156-9.
[3] Maniatis T, Fritsch EF, Sambrook J. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Lab, 1982; 545 p.
[4] Haughland RP. Molecular probes. Handbook of fluorescent probes and research chemicals. Eugene, OR., 1996. 679 p.
[5] Lee LG, Chen CH, Chiu LA. Thiazole orange: a new dye for reticulocyte analysis. Cytometry. 1986;7(6):508-17.
[6] Yarmoluk SM, Zhyvoloup AN, Koval'ska VB, Klimenko IV, Kukharenko AP, Kovtun YP, Slominsky YL. Interaction of cyanine dyes with nucleic acids. I. Studies on monomethyne cyanine dyes as possible fluorescent probes for the detection of nucleic acids. Biopolym Cell. 1996; 12(1):69-74.
[7] Yarmoluk SM, Kovalska VB, Smirnova TV, Shandura MP, Kovtun YP, Matsuka GKh. Interaction of cyanine dyes with nucleic acids. 2. Spectroscopic properties of methyleneoxy analogues of Thiazole Orange. Biopolym Cell. 1996; 12(6):74-81.